Silicon and Ralstonia solanacearum modulate expression stability of housekeeping genes in tomato

Hassan Ghareeb, Zoltán Bozsó, P. Ott, Kerstin Wydra

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

For analysis of gene expression profiles underlying tomato-Ralstonia solanacearum interaction and silicon-induced resistance, an internal control with high expression stability under the experimental conditions is needed as a prerequisite for accurate relative quantification of gene expression. Therefore, the expression stability of three housekeeping genes, phosphoglycerate kinase genes (PGK), α-tubulin (TUB) and actin (ACT) in tomato under 24 experimental conditions including combinations of silicon amendments and inoculation with R. solanacearum at six time points was examined. The expression of ACT severely varied, in particular at the early phase after inoculation with the pathogen, suggesting a possible disabling the cytoskeleton that mediates resistance. Application of silicon resulted in more expression stability of the three housekeeping genes, showing alleviation of the biotic stress imposed by the pathogen. Although PGK and TUB showed reasonable expression stability, PGK was frequently ranked as the more stable among the treatments and over time. Thus, it is misleading to use a commonly used housekeeping gene for normalization without prior investigation of its expression stability. Here, we recommend to use either PGK alone, or PGK and TUB together as internal controls to increase the accuracy and to avoid error propagation in quantification of relative gene expression.

Original languageEnglish
Pages (from-to)176-179
Number of pages4
JournalPhysiological and Molecular Plant Pathology
Volume75
Issue number4
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Ralstonia solanacearum
phosphoglycerate kinase
Phosphoglycerate Kinase
Essential Genes
Silicon
Lycopersicon esculentum
silicon
tomatoes
Tubulin
tubulin
Genes
genes
gene expression
actin
Actins
Gene Expression
pathogens
induced resistance
biotic stress
cytoskeleton

Keywords

  • α-Tubulin
  • Actin
  • Gene expression stability
  • Phosphoglycerate kinase gene
  • Ralstonia solanacearum
  • Silicon
  • Solanum lycopersicum

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Silicon and Ralstonia solanacearum modulate expression stability of housekeeping genes in tomato. / Ghareeb, Hassan; Bozsó, Zoltán; Ott, P.; Wydra, Kerstin.

In: Physiological and Molecular Plant Pathology, Vol. 75, No. 4, 04.2011, p. 176-179.

Research output: Contribution to journalArticle

@article{93e290b7560e493b85efc36b8b2bf021,
title = "Silicon and Ralstonia solanacearum modulate expression stability of housekeeping genes in tomato",
abstract = "For analysis of gene expression profiles underlying tomato-Ralstonia solanacearum interaction and silicon-induced resistance, an internal control with high expression stability under the experimental conditions is needed as a prerequisite for accurate relative quantification of gene expression. Therefore, the expression stability of three housekeeping genes, phosphoglycerate kinase genes (PGK), α-tubulin (TUB) and actin (ACT) in tomato under 24 experimental conditions including combinations of silicon amendments and inoculation with R. solanacearum at six time points was examined. The expression of ACT severely varied, in particular at the early phase after inoculation with the pathogen, suggesting a possible disabling the cytoskeleton that mediates resistance. Application of silicon resulted in more expression stability of the three housekeeping genes, showing alleviation of the biotic stress imposed by the pathogen. Although PGK and TUB showed reasonable expression stability, PGK was frequently ranked as the more stable among the treatments and over time. Thus, it is misleading to use a commonly used housekeeping gene for normalization without prior investigation of its expression stability. Here, we recommend to use either PGK alone, or PGK and TUB together as internal controls to increase the accuracy and to avoid error propagation in quantification of relative gene expression.",
keywords = "α-Tubulin, Actin, Gene expression stability, Phosphoglycerate kinase gene, Ralstonia solanacearum, Silicon, Solanum lycopersicum",
author = "Hassan Ghareeb and Zolt{\'a}n Bozs{\'o} and P. Ott and Kerstin Wydra",
year = "2011",
month = "4",
doi = "10.1016/j.pmpp.2011.02.003",
language = "English",
volume = "75",
pages = "176--179",
journal = "Physiological and Molecular Plant Pathology",
issn = "0885-5765",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Silicon and Ralstonia solanacearum modulate expression stability of housekeeping genes in tomato

AU - Ghareeb, Hassan

AU - Bozsó, Zoltán

AU - Ott, P.

AU - Wydra, Kerstin

PY - 2011/4

Y1 - 2011/4

N2 - For analysis of gene expression profiles underlying tomato-Ralstonia solanacearum interaction and silicon-induced resistance, an internal control with high expression stability under the experimental conditions is needed as a prerequisite for accurate relative quantification of gene expression. Therefore, the expression stability of three housekeeping genes, phosphoglycerate kinase genes (PGK), α-tubulin (TUB) and actin (ACT) in tomato under 24 experimental conditions including combinations of silicon amendments and inoculation with R. solanacearum at six time points was examined. The expression of ACT severely varied, in particular at the early phase after inoculation with the pathogen, suggesting a possible disabling the cytoskeleton that mediates resistance. Application of silicon resulted in more expression stability of the three housekeeping genes, showing alleviation of the biotic stress imposed by the pathogen. Although PGK and TUB showed reasonable expression stability, PGK was frequently ranked as the more stable among the treatments and over time. Thus, it is misleading to use a commonly used housekeeping gene for normalization without prior investigation of its expression stability. Here, we recommend to use either PGK alone, or PGK and TUB together as internal controls to increase the accuracy and to avoid error propagation in quantification of relative gene expression.

AB - For analysis of gene expression profiles underlying tomato-Ralstonia solanacearum interaction and silicon-induced resistance, an internal control with high expression stability under the experimental conditions is needed as a prerequisite for accurate relative quantification of gene expression. Therefore, the expression stability of three housekeeping genes, phosphoglycerate kinase genes (PGK), α-tubulin (TUB) and actin (ACT) in tomato under 24 experimental conditions including combinations of silicon amendments and inoculation with R. solanacearum at six time points was examined. The expression of ACT severely varied, in particular at the early phase after inoculation with the pathogen, suggesting a possible disabling the cytoskeleton that mediates resistance. Application of silicon resulted in more expression stability of the three housekeeping genes, showing alleviation of the biotic stress imposed by the pathogen. Although PGK and TUB showed reasonable expression stability, PGK was frequently ranked as the more stable among the treatments and over time. Thus, it is misleading to use a commonly used housekeeping gene for normalization without prior investigation of its expression stability. Here, we recommend to use either PGK alone, or PGK and TUB together as internal controls to increase the accuracy and to avoid error propagation in quantification of relative gene expression.

KW - α-Tubulin

KW - Actin

KW - Gene expression stability

KW - Phosphoglycerate kinase gene

KW - Ralstonia solanacearum

KW - Silicon

KW - Solanum lycopersicum

UR - http://www.scopus.com/inward/record.url?scp=79958009986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958009986&partnerID=8YFLogxK

U2 - 10.1016/j.pmpp.2011.02.003

DO - 10.1016/j.pmpp.2011.02.003

M3 - Article

VL - 75

SP - 176

EP - 179

JO - Physiological and Molecular Plant Pathology

JF - Physiological and Molecular Plant Pathology

SN - 0885-5765

IS - 4

ER -