Silencing the KCNK9 potassium channel (TASK-3) gene disturbs mitochondrial function, causes mitochondrial depolarization, and induces apoptosis of human melanoma cells

Dénes Nagy, M. Gönczi, Beatrix Dienes, Árpád Szöőr, János Fodor, Zsuzsanna Nagy, Adrienn Tóth, Tamás Fodor, P. Bai, G. Szücs, Zoltán Rusznák, L. Csernoch

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

TASK-3 (KCNK9 or K2P9.1) channels are thought to promote proliferation and/or survival of malignantly transformed cells, most likely by increasing their hypoxia tolerance. Based on our previous results that suggested mitochondrial expression of TASK-3 channels, we hypothesized that TASK-3 channels have roles in maintaining mitochondrial activity. In the present work we studied the effect of reduced TASK-3 expression on the mitochondrial function and survival of WM35 and A2058 melanoma cells. TASK-3 knockdown cells had depolarized mitochondrial membrane potential and contained a reduced amount of mitochondrial DNA. Compared to their scrambled shRNA-transfected counterparts, they demonstrated diminished responsiveness to the application of the mitochondrial uncoupler [(3-chlorophenyl)hydrazono]malononitrile (CCCP). These observations indicate impaired mitochondrial function. Further, TASK-3 knockdown cells presented reduced viability, decreased total DNA content, altered cell morphology, and reduced surface area. In contrast to non- and scrambled shRNA-transfected melanoma cell lines, which did not present noteworthy apoptotic activity, almost 50 % of the TASK-3 knockdown cells exhibited strong Annexin-V-specific immunofluorescence signal. Sequestration of cytochrome c from the mitochondria to the cytosol, increased caspase 3 activity, and translocation of the apoptosis-inducing factor from mitochondria to cell nuclei were also demonstrated in TASK-3 knockdown cells. Interference with TASK-3 channel expression, therefore, induces caspase-dependent and -independent apoptosis of melanoma cells, most likely via causing mitochondrial depolarization. Consequently, TASK-3 channels may be legitimate targets of future melanoma therapies.

Original languageEnglish
Pages (from-to)885-902
Number of pages18
JournalArchives of Dermatological Research
Volume306
Issue number10
DOIs
Publication statusPublished - 2014

Fingerprint

Mitochondrial Genes
Potassium Channels
Melanoma
Apoptosis
Small Interfering RNA
Mitochondria
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Apoptosis Inducing Factor
Survival
Mitochondrial Membrane Potential
Annexin A5
Caspases
Cytochromes c
Cell Nucleus
Mitochondrial DNA
Caspase 3
Cytosol
Fluorescent Antibody Technique
Cell Line
DNA

Keywords

  • A2058 melanoma cells
  • Caspase 3
  • CCCP
  • Cytochrome c
  • JC-1
  • Mitochondrial membrane potential
  • Mitotracker
  • TASK-3 knockdown
  • WM35 melanoma cells

ASJC Scopus subject areas

  • Dermatology
  • Medicine(all)

Cite this

Silencing the KCNK9 potassium channel (TASK-3) gene disturbs mitochondrial function, causes mitochondrial depolarization, and induces apoptosis of human melanoma cells. / Nagy, Dénes; Gönczi, M.; Dienes, Beatrix; Szöőr, Árpád; Fodor, János; Nagy, Zsuzsanna; Tóth, Adrienn; Fodor, Tamás; Bai, P.; Szücs, G.; Rusznák, Zoltán; Csernoch, L.

In: Archives of Dermatological Research, Vol. 306, No. 10, 2014, p. 885-902.

Research output: Contribution to journalArticle

Nagy, Dénes ; Gönczi, M. ; Dienes, Beatrix ; Szöőr, Árpád ; Fodor, János ; Nagy, Zsuzsanna ; Tóth, Adrienn ; Fodor, Tamás ; Bai, P. ; Szücs, G. ; Rusznák, Zoltán ; Csernoch, L. / Silencing the KCNK9 potassium channel (TASK-3) gene disturbs mitochondrial function, causes mitochondrial depolarization, and induces apoptosis of human melanoma cells. In: Archives of Dermatological Research. 2014 ; Vol. 306, No. 10. pp. 885-902.
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AU - Nagy, Dénes

AU - Gönczi, M.

AU - Dienes, Beatrix

AU - Szöőr, Árpád

AU - Fodor, János

AU - Nagy, Zsuzsanna

AU - Tóth, Adrienn

AU - Fodor, Tamás

AU - Bai, P.

AU - Szücs, G.

AU - Rusznák, Zoltán

AU - Csernoch, L.

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AB - TASK-3 (KCNK9 or K2P9.1) channels are thought to promote proliferation and/or survival of malignantly transformed cells, most likely by increasing their hypoxia tolerance. Based on our previous results that suggested mitochondrial expression of TASK-3 channels, we hypothesized that TASK-3 channels have roles in maintaining mitochondrial activity. In the present work we studied the effect of reduced TASK-3 expression on the mitochondrial function and survival of WM35 and A2058 melanoma cells. TASK-3 knockdown cells had depolarized mitochondrial membrane potential and contained a reduced amount of mitochondrial DNA. Compared to their scrambled shRNA-transfected counterparts, they demonstrated diminished responsiveness to the application of the mitochondrial uncoupler [(3-chlorophenyl)hydrazono]malononitrile (CCCP). These observations indicate impaired mitochondrial function. Further, TASK-3 knockdown cells presented reduced viability, decreased total DNA content, altered cell morphology, and reduced surface area. In contrast to non- and scrambled shRNA-transfected melanoma cell lines, which did not present noteworthy apoptotic activity, almost 50 % of the TASK-3 knockdown cells exhibited strong Annexin-V-specific immunofluorescence signal. Sequestration of cytochrome c from the mitochondria to the cytosol, increased caspase 3 activity, and translocation of the apoptosis-inducing factor from mitochondria to cell nuclei were also demonstrated in TASK-3 knockdown cells. Interference with TASK-3 channel expression, therefore, induces caspase-dependent and -independent apoptosis of melanoma cells, most likely via causing mitochondrial depolarization. Consequently, TASK-3 channels may be legitimate targets of future melanoma therapies.

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