Silencing SERCA1b in a few fibers stimulates growth in the entire regenerating soleus muscle

E. Zádor, Grzegorz Owsianik, Frank Wuytack

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The neonatal isoform of the sarcoplasmic/endoplasmic reticulum Ca 2+ ATPase 1 (SERCA1b) is a dominant Ca2+ pump in the young fibers of regenerating muscle. In vivo transfection of about 1% of the fibers with SERCA1b RNAi plasmid resulted in no apparent change in the transfected fibers, but enhanced the increase of fresh weight and fiber size in the whole regenerating rat soleus muscle, until the normal size was reached. Co-transfection of calcineurin inhibitor cain/cabin-1 with SERCA1b RNAi was sufficient to cut down the widespread growth stimulation, but the subsequent transfection of cain into the SERCA1b RNAi transfected muscle did not inhibit muscle growth. The SERCA1b RNAi preferably upregulated the expression of the NFAT reporter lacZ compared to controls when co-transfected into the fibers. Notably, perimuscular injection of interleukin-4 (IL-4) antibody but not that of an unrelevant antibody completely abolished the growth-promoting effect of SERCA1b RNAi. This indicates that silencing SERCA1b in a few fibers stimulates the calcineurin-NFAT-IL-4 pathway and fiber growth in the whole regenerating soleus. These results suggest the presence of an autocrine-paracrine coordination of growing muscle fibers, and put forward a new method to stimulate skeletal muscle regeneration.

Original languageEnglish
Pages (from-to)11-20
Number of pages10
JournalHistochemistry and Cell Biology
Volume135
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

RNA Interference
Skeletal Muscle
Transfection
Growth
Muscles
Interleukin-4
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Antibodies
Calcineurin
Endoplasmic Reticulum
Regeneration
Protein Isoforms
Plasmids
Weights and Measures
Injections

Keywords

  • Calcineurin
  • Fiber growth
  • Interleukin-4
  • Muscle regeneration
  • SERCA1b

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Medical Laboratory Technology
  • Molecular Biology

Cite this

Silencing SERCA1b in a few fibers stimulates growth in the entire regenerating soleus muscle. / Zádor, E.; Owsianik, Grzegorz; Wuytack, Frank.

In: Histochemistry and Cell Biology, Vol. 135, No. 1, 01.2011, p. 11-20.

Research output: Contribution to journalArticle

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