Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance

A. Galambos, A. Zok, A. Kuczmog, R. Oláh, P. Putnoky, W. Ream, E. Szegedi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Key message: Grapevine rootstock transformed with an Agrobacterium oncogene-silencing transgene was resistant to certain Agrobacterium strains but sensitive to others. Thus, genetic diversity of Agrobacterium oncogenes may limit engineering crown gall resistance. Crown gall disease of grapevine induced by Agrobacterium vitis or Agrobacterium tumefaciens causes serious economic losses in viticulture. To establish crown gall-resistant lines, somatic proembryos of Vitis berlandieri × V. rupestris cv. 'Richter 110' rootstock were transformed with an oncogene-silencing transgene based on iaaM and ipt oncogene sequences from octopine-type, tumor-inducing (Ti) plasmid pTiA6. Twenty-one transgenic lines were selected, and their transgenic nature was confirmed by polymerase chain reaction (PCR). These lines were inoculated with two A. tumefaciens and three A. vitis strains. Eight lines showed resistance to octopine-type A. tumefaciens A348. Resistance correlated with the expression of the silencing genes. However, oncogene silencing was mostly sequence specific because these lines did not abolish tumorigenesis by A. vitis strains or nopaline-type A. tumefaciens C58.

Original languageEnglish
Pages (from-to)1751-1757
Number of pages7
JournalPlant Cell Reports
Volume32
Issue number11
DOIs
Publication statusPublished - Nov 2013

Fingerprint

crown galls
oncogenes
Agrobacterium
Rhizobium vitis
Agrobacterium radiobacter
genetically modified organisms
octopine
transgenes
rootstocks
Vitis cinerea var. helleri
Vitis rupestris
nopaline
viticulture
gene silencing
carcinogenesis
plasmids
engineering
polymerase chain reaction
economics
genetic variation

Keywords

  • Agrobacterium tumefaciens
  • Agrobacterium vitis
  • Crown gall
  • Transgenic grapevine
  • Vitis berlandieri × V. rupestris cv. 'Richter 110'

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance. / Galambos, A.; Zok, A.; Kuczmog, A.; Oláh, R.; Putnoky, P.; Ream, W.; Szegedi, E.

In: Plant Cell Reports, Vol. 32, No. 11, 11.2013, p. 1751-1757.

Research output: Contribution to journalArticle

Galambos, A. ; Zok, A. ; Kuczmog, A. ; Oláh, R. ; Putnoky, P. ; Ream, W. ; Szegedi, E. / Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance. In: Plant Cell Reports. 2013 ; Vol. 32, No. 11. pp. 1751-1757.
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