Sildenafil, N-desmethyl-sildenafil and Zaprinast enhance photoreceptor response in the isolated rat retina

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3 Citations (Scopus)

Abstract

Here we report that the active component of Viagra, Sildenafil and the first metabolite, N-desmethyl-sildenafil (UK-103, 320) increased the amplitude of flash-evoked electroretinogram (ERG) of dark-adapted albino rat retina. Effects of Sildenafil and N-desmethyl-sildenafil were comparable to those of the known phosphodiesterase inhibitor, Zaprinast. The photoreceptor cell response was isolated by blocking the glial K+ ion-buffering and the on-bipolar components of the ERG with the use of BaCl2 (500μM) and the specific type VI metabotropic glutamate receptor agonist, DL-2-amino-4-phosphonobutyric acid (25μM), respectively. Zaprinast, Sildenafil and N-desmethyl-sildenafil (1μM each) increased the amplitude of photoreceptor cell response either. Besides, Sildenafil was significantly more effective than N-desmethyl-sildenafil. These findings suggest an increased sensitivity of photoreceptor cells in the presence of Sildenafil and it is metabolite.

Original languageEnglish
Pages (from-to)591-595
Number of pages5
JournalNeurochemistry international
Volume43
Issue number6
DOIs
Publication statusPublished - Nov 2003

Keywords

  • DL-2-amino-4-phosphonobutyric acid
  • ERG
  • N-desmethyl-sildenafil
  • Phosphodiesterase inhibitors
  • Photoresponse
  • Rat retina
  • Sildenafil
  • Zaprinast

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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