Sigma and opioid receptors in human brain tumors

Gail E. Thomas, M. Szücs, James Y. Mamone, Wojciech T. Bem, Michael D. Rush, Frank E. Johnson, Carmine J. Coscia

Research output: Contribution to journalArticle

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Abstract

Human brain tumors (obtained as surgical specimens) and nude mouse-borne human neuroblastomas and gliomas were analyzed for sigma and opioid receptor content. Sigma binding was assessed using [3H] 1, 3-di-o-tolylguanidine (DTG), whereas opioid receptor subtypes were measured with tritiated forms of the following: μ, [D-ala2, mePhe4, gly-ol5] enkephalin (DAMGE); κ, ethylketocyclazocine (EKC) or U69, 593; δ, [D-pen2, D-pen5] enkephalin (DPDPE) or [D-ala2, D-leu5] enkephalin (DADLE) with μ suppressor present. Binding parameters were estimated by homologous displacement assays followed by analysis using the LIGAND program. Sigma binding was detected in 15 of 16 tumors examined with very high levels (pmol/mg protein) found in a brain metastasis from an adenocarcinoma of lung and a human neuroblastoma (SK-N-MC) passaged in nude mice. κ opioid receptor binding was detected in 4 of 4 glioblastoma multiforme specimens and 2 of 2 human astrocytoma cell lines tested but not in the other brain tumors analyzed.

Original languageEnglish
Pages (from-to)1279-1286
Number of pages8
JournalLife Sciences
Volume46
Issue number18
DOIs
Publication statusPublished - 1990

Fingerprint

sigma Receptors
Brain Neoplasms
Tumors
Brain
Enkephalins
Opioid Receptors
Ethylketocyclazocine
Neuroblastoma
Nude Mice
D-Penicillamine (2,5)-Enkephalin
Assays
Astrocytoma
Glioblastoma
Cells
Glioma
Neoplasm Metastasis
Cell Line
Proteins
Neoplasms

ASJC Scopus subject areas

  • Pharmacology

Cite this

Thomas, G. E., Szücs, M., Mamone, J. Y., Bem, W. T., Rush, M. D., Johnson, F. E., & Coscia, C. J. (1990). Sigma and opioid receptors in human brain tumors. Life Sciences, 46(18), 1279-1286. https://doi.org/10.1016/0024-3205(90)90360-4

Sigma and opioid receptors in human brain tumors. / Thomas, Gail E.; Szücs, M.; Mamone, James Y.; Bem, Wojciech T.; Rush, Michael D.; Johnson, Frank E.; Coscia, Carmine J.

In: Life Sciences, Vol. 46, No. 18, 1990, p. 1279-1286.

Research output: Contribution to journalArticle

Thomas, GE, Szücs, M, Mamone, JY, Bem, WT, Rush, MD, Johnson, FE & Coscia, CJ 1990, 'Sigma and opioid receptors in human brain tumors', Life Sciences, vol. 46, no. 18, pp. 1279-1286. https://doi.org/10.1016/0024-3205(90)90360-4
Thomas GE, Szücs M, Mamone JY, Bem WT, Rush MD, Johnson FE et al. Sigma and opioid receptors in human brain tumors. Life Sciences. 1990;46(18):1279-1286. https://doi.org/10.1016/0024-3205(90)90360-4
Thomas, Gail E. ; Szücs, M. ; Mamone, James Y. ; Bem, Wojciech T. ; Rush, Michael D. ; Johnson, Frank E. ; Coscia, Carmine J. / Sigma and opioid receptors in human brain tumors. In: Life Sciences. 1990 ; Vol. 46, No. 18. pp. 1279-1286.
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