Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry

Nicole Strittmatter, Anna Lovrics, Judit Sessler, James S. McKenzie, Zsolt Bodai, M. Luisa Doria, Nora Kucsma, G. Szakács, Zoltan Takats

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Rapid evaporative ionization mass spectrometry (REIMS) was used for the rapid mass spectrometric profiling of cancer cell lines. Spectral reproducibility was assessed for three different cell lines, and the extent of interclass differences and intraclass variance was found to allow the identification of these cell lines based on the REIMS data. Subsequently, the NCI60 cell line panel was subjected to REIMS analysis, and the resulting data set was investigated for its distinction of individual cell lines and different tissue types of origin. Information content of REIMS spectral profiles of cell lines were found to be similar to those obtained from mammalian tissues although pronounced differences in relative lipid intensity were observed. Ultimately, REIMS was shown to detect changes in lipid content of cell lines due to mycoplasma infection. The data show that REIMS is an attractive means to study cell lines involving minimal sample preparation and analysis times in the range of seconds.

Original languageEnglish
Pages (from-to)7507-7514
Number of pages8
JournalAnalytical Chemistry
Volume88
Issue number15
DOIs
Publication statusPublished - Aug 2 2016

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Ionization
Mass spectrometry
Cells
Tissue
Lipids

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Strittmatter, N., Lovrics, A., Sessler, J., McKenzie, J. S., Bodai, Z., Doria, M. L., ... Takats, Z. (2016). Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry. Analytical Chemistry, 88(15), 7507-7514. https://doi.org/10.1021/acs.analchem.6b00187

Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry. / Strittmatter, Nicole; Lovrics, Anna; Sessler, Judit; McKenzie, James S.; Bodai, Zsolt; Doria, M. Luisa; Kucsma, Nora; Szakács, G.; Takats, Zoltan.

In: Analytical Chemistry, Vol. 88, No. 15, 02.08.2016, p. 7507-7514.

Research output: Contribution to journalArticle

Strittmatter, N, Lovrics, A, Sessler, J, McKenzie, JS, Bodai, Z, Doria, ML, Kucsma, N, Szakács, G & Takats, Z 2016, 'Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry', Analytical Chemistry, vol. 88, no. 15, pp. 7507-7514. https://doi.org/10.1021/acs.analchem.6b00187
Strittmatter, Nicole ; Lovrics, Anna ; Sessler, Judit ; McKenzie, James S. ; Bodai, Zsolt ; Doria, M. Luisa ; Kucsma, Nora ; Szakács, G. ; Takats, Zoltan. / Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry. In: Analytical Chemistry. 2016 ; Vol. 88, No. 15. pp. 7507-7514.
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