Short-term effects of T-2 toxin or deoxynivalenol on glutathione status and expression of its regulatory genes in chicken

Mangesh Nakade, Csilla Pelyhe, Benjámin Kövesi, K. Balogh, Balázs Kovács, Judit Szabó-Fodor, Erika Zándoki, M. Mézes, Márta Erdélyi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Short-term (48-hour) effects of 3.74/1.26 mg kg-1 T-2/HT-2 toxin or 16.12 mg kg-1 DON in feed were investigated in the liver of three-week-old cockerels (body weight: 749.60 ± 90.98 g). Markers of lipid peroxidation showed no significant changes. At hour 24, glutathione content in the T-2/HT-2 toxin group was significantly higher than in the control. Glutathione peroxidase activity was significantly higher than the control at hour 24 in the T-2/H-2 toxin group and at hour 48 in the DON group. In the DON group, expression of the glutathione peroxidase 4 gene (GPX4) was significantly lower than in the control at hours 12 and 14, and higher at hour 48. Expression of the glutathione reductase gene (GSR) was significantly lower than in the control at hour 12 in the T-2/HT-2 toxin group, and at hours 12, 24 and 48 in the DON group. However, at hour 36 higher GSR expression was measured in the DON group. Due to the effect of both trichothecenes, expression of the glutathione synthetase gene (GSS) was significantly lower than in the control at hours 24 and 48. In conclusion, T-2/HT-2 toxin and DON had a moderate short-term effect on free radical formation. T-2/HT-2 toxin induced more pronounced activation of the glutathione redox system than did DON.

Original languageEnglish
Pages (from-to)28-39
Number of pages12
JournalActa Veterinaria Hungarica
Volume66
Issue number1
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

T-2 Toxin
HT-2 toxin
T-2 toxin
deoxynivalenol
Regulator Genes
regulator genes
Glutathione
glutathione
Chickens
chickens
phospholipid-hydroperoxide glutathione peroxidase
Glutathione Synthase
glutathione synthase
Trichothecenes
Genes
cockerels
trichothecenes
genes
Glutathione Reductase
Glutathione Peroxidase

Keywords

  • Chicken
  • Deoxynivalenol
  • Glutathione peroxidase
  • Gpx genes
  • T-2/HT-2 toxin

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Short-term effects of T-2 toxin or deoxynivalenol on glutathione status and expression of its regulatory genes in chicken. / Nakade, Mangesh; Pelyhe, Csilla; Kövesi, Benjámin; Balogh, K.; Kovács, Balázs; Szabó-Fodor, Judit; Zándoki, Erika; Mézes, M.; Erdélyi, Márta.

In: Acta Veterinaria Hungarica, Vol. 66, No. 1, 01.03.2018, p. 28-39.

Research output: Contribution to journalArticle

Nakade, Mangesh ; Pelyhe, Csilla ; Kövesi, Benjámin ; Balogh, K. ; Kovács, Balázs ; Szabó-Fodor, Judit ; Zándoki, Erika ; Mézes, M. ; Erdélyi, Márta. / Short-term effects of T-2 toxin or deoxynivalenol on glutathione status and expression of its regulatory genes in chicken. In: Acta Veterinaria Hungarica. 2018 ; Vol. 66, No. 1. pp. 28-39.
@article{8040c7a84e764b70879838f4bc8d1983,
title = "Short-term effects of T-2 toxin or deoxynivalenol on glutathione status and expression of its regulatory genes in chicken",
abstract = "Short-term (48-hour) effects of 3.74/1.26 mg kg-1 T-2/HT-2 toxin or 16.12 mg kg-1 DON in feed were investigated in the liver of three-week-old cockerels (body weight: 749.60 ± 90.98 g). Markers of lipid peroxidation showed no significant changes. At hour 24, glutathione content in the T-2/HT-2 toxin group was significantly higher than in the control. Glutathione peroxidase activity was significantly higher than the control at hour 24 in the T-2/H-2 toxin group and at hour 48 in the DON group. In the DON group, expression of the glutathione peroxidase 4 gene (GPX4) was significantly lower than in the control at hours 12 and 14, and higher at hour 48. Expression of the glutathione reductase gene (GSR) was significantly lower than in the control at hour 12 in the T-2/HT-2 toxin group, and at hours 12, 24 and 48 in the DON group. However, at hour 36 higher GSR expression was measured in the DON group. Due to the effect of both trichothecenes, expression of the glutathione synthetase gene (GSS) was significantly lower than in the control at hours 24 and 48. In conclusion, T-2/HT-2 toxin and DON had a moderate short-term effect on free radical formation. T-2/HT-2 toxin induced more pronounced activation of the glutathione redox system than did DON.",
keywords = "Chicken, Deoxynivalenol, Glutathione peroxidase, Gpx genes, T-2/HT-2 toxin",
author = "Mangesh Nakade and Csilla Pelyhe and Benj{\'a}min K{\"o}vesi and K. Balogh and Bal{\'a}zs Kov{\'a}cs and Judit Szab{\'o}-Fodor and Erika Z{\'a}ndoki and M. M{\'e}zes and M{\'a}rta Erd{\'e}lyi",
year = "2018",
month = "3",
day = "1",
doi = "10.1556/004.2018.004",
language = "English",
volume = "66",
pages = "28--39",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Short-term effects of T-2 toxin or deoxynivalenol on glutathione status and expression of its regulatory genes in chicken

AU - Nakade, Mangesh

AU - Pelyhe, Csilla

AU - Kövesi, Benjámin

AU - Balogh, K.

AU - Kovács, Balázs

AU - Szabó-Fodor, Judit

AU - Zándoki, Erika

AU - Mézes, M.

AU - Erdélyi, Márta

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Short-term (48-hour) effects of 3.74/1.26 mg kg-1 T-2/HT-2 toxin or 16.12 mg kg-1 DON in feed were investigated in the liver of three-week-old cockerels (body weight: 749.60 ± 90.98 g). Markers of lipid peroxidation showed no significant changes. At hour 24, glutathione content in the T-2/HT-2 toxin group was significantly higher than in the control. Glutathione peroxidase activity was significantly higher than the control at hour 24 in the T-2/H-2 toxin group and at hour 48 in the DON group. In the DON group, expression of the glutathione peroxidase 4 gene (GPX4) was significantly lower than in the control at hours 12 and 14, and higher at hour 48. Expression of the glutathione reductase gene (GSR) was significantly lower than in the control at hour 12 in the T-2/HT-2 toxin group, and at hours 12, 24 and 48 in the DON group. However, at hour 36 higher GSR expression was measured in the DON group. Due to the effect of both trichothecenes, expression of the glutathione synthetase gene (GSS) was significantly lower than in the control at hours 24 and 48. In conclusion, T-2/HT-2 toxin and DON had a moderate short-term effect on free radical formation. T-2/HT-2 toxin induced more pronounced activation of the glutathione redox system than did DON.

AB - Short-term (48-hour) effects of 3.74/1.26 mg kg-1 T-2/HT-2 toxin or 16.12 mg kg-1 DON in feed were investigated in the liver of three-week-old cockerels (body weight: 749.60 ± 90.98 g). Markers of lipid peroxidation showed no significant changes. At hour 24, glutathione content in the T-2/HT-2 toxin group was significantly higher than in the control. Glutathione peroxidase activity was significantly higher than the control at hour 24 in the T-2/H-2 toxin group and at hour 48 in the DON group. In the DON group, expression of the glutathione peroxidase 4 gene (GPX4) was significantly lower than in the control at hours 12 and 14, and higher at hour 48. Expression of the glutathione reductase gene (GSR) was significantly lower than in the control at hour 12 in the T-2/HT-2 toxin group, and at hours 12, 24 and 48 in the DON group. However, at hour 36 higher GSR expression was measured in the DON group. Due to the effect of both trichothecenes, expression of the glutathione synthetase gene (GSS) was significantly lower than in the control at hours 24 and 48. In conclusion, T-2/HT-2 toxin and DON had a moderate short-term effect on free radical formation. T-2/HT-2 toxin induced more pronounced activation of the glutathione redox system than did DON.

KW - Chicken

KW - Deoxynivalenol

KW - Glutathione peroxidase

KW - Gpx genes

KW - T-2/HT-2 toxin

UR - http://www.scopus.com/inward/record.url?scp=85045835575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045835575&partnerID=8YFLogxK

U2 - 10.1556/004.2018.004

DO - 10.1556/004.2018.004

M3 - Article

C2 - 29580086

AN - SCOPUS:85045835575

VL - 66

SP - 28

EP - 39

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 1

ER -