Short-term effects of N-acetylcysteine and ischemic preconditioning in a canine model of hepatic ischemia-reperfusion injury

J. Baumann, S. Ghosh, T. Szakmany, G. Jancso, A. Ferencz, E. Rőth, L. Bogár

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aims: We evaluated the possibility that repeated ischemic preconditioning or N-acetylcysteine (NAC) could prevent ischemia-reperfusion injury as determined by indocyanine green plasma disappearance rate (ICG-PDR) or has favorable hemodynamic effects during reperfusion in an in vivo canine liver model. Methods: Under general anesthesia, 3 groups of mongrel dogs (n = 5 per group) were subjected to (1) 60-min hepatic ischemia, (2) same ischemia preceded by intravenous administration of 150 mg kg-1 NAC, and (3) three episodes of IPC (10-min ischemia followed by 10-min reperfusion) prior to same ischemia. Hepatic reperfusion was maintained for a further 180 min, with hemodynamic and hepatic function parameters monitored throughout. Results: Plasma disappearance rate of indocyanine green and serum levels of aspartate transferase and alanine transferase showed no significant differences between groups. Although liver injury was obvious, reflected by hemodynamic, blood gas, and liver function tests, NAC and IPC failed to prevent decay in hepatic function in this canine model. Conclusion: The results do not support the hypothesis that short-term use of NAC and IPC is beneficial in hepatic surgery.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalEuropean Surgical Research
Volume41
Issue number2
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Ischemic Preconditioning
Acetylcysteine
Reperfusion Injury
Canidae
Liver
Ischemia
Reperfusion
Indocyanine Green
Hemodynamics
Transferases
Liver Function Tests
Aspartic Acid
Alanine
Intravenous Administration
General Anesthesia
Gases
Dogs
Wounds and Injuries
Serum

Keywords

  • Hemodynamics
  • Ischemia-reperfusion injury
  • N-acetylcysteine
  • Preconditioning

ASJC Scopus subject areas

  • Surgery

Cite this

Short-term effects of N-acetylcysteine and ischemic preconditioning in a canine model of hepatic ischemia-reperfusion injury. / Baumann, J.; Ghosh, S.; Szakmany, T.; Jancso, G.; Ferencz, A.; Rőth, E.; Bogár, L.

In: European Surgical Research, Vol. 41, No. 2, 08.2008, p. 226-230.

Research output: Contribution to journalArticle

@article{c65b7fad85d344128372b220d7d114c7,
title = "Short-term effects of N-acetylcysteine and ischemic preconditioning in a canine model of hepatic ischemia-reperfusion injury",
abstract = "Aims: We evaluated the possibility that repeated ischemic preconditioning or N-acetylcysteine (NAC) could prevent ischemia-reperfusion injury as determined by indocyanine green plasma disappearance rate (ICG-PDR) or has favorable hemodynamic effects during reperfusion in an in vivo canine liver model. Methods: Under general anesthesia, 3 groups of mongrel dogs (n = 5 per group) were subjected to (1) 60-min hepatic ischemia, (2) same ischemia preceded by intravenous administration of 150 mg kg-1 NAC, and (3) three episodes of IPC (10-min ischemia followed by 10-min reperfusion) prior to same ischemia. Hepatic reperfusion was maintained for a further 180 min, with hemodynamic and hepatic function parameters monitored throughout. Results: Plasma disappearance rate of indocyanine green and serum levels of aspartate transferase and alanine transferase showed no significant differences between groups. Although liver injury was obvious, reflected by hemodynamic, blood gas, and liver function tests, NAC and IPC failed to prevent decay in hepatic function in this canine model. Conclusion: The results do not support the hypothesis that short-term use of NAC and IPC is beneficial in hepatic surgery.",
keywords = "Hemodynamics, Ischemia-reperfusion injury, N-acetylcysteine, Preconditioning",
author = "J. Baumann and S. Ghosh and T. Szakmany and G. Jancso and A. Ferencz and E. Rőth and L. Bog{\'a}r",
year = "2008",
month = "8",
doi = "10.1159/000135707",
language = "English",
volume = "41",
pages = "226--230",
journal = "European Surgical Research",
issn = "0014-312X",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Short-term effects of N-acetylcysteine and ischemic preconditioning in a canine model of hepatic ischemia-reperfusion injury

AU - Baumann, J.

AU - Ghosh, S.

AU - Szakmany, T.

AU - Jancso, G.

AU - Ferencz, A.

AU - Rőth, E.

AU - Bogár, L.

PY - 2008/8

Y1 - 2008/8

N2 - Aims: We evaluated the possibility that repeated ischemic preconditioning or N-acetylcysteine (NAC) could prevent ischemia-reperfusion injury as determined by indocyanine green plasma disappearance rate (ICG-PDR) or has favorable hemodynamic effects during reperfusion in an in vivo canine liver model. Methods: Under general anesthesia, 3 groups of mongrel dogs (n = 5 per group) were subjected to (1) 60-min hepatic ischemia, (2) same ischemia preceded by intravenous administration of 150 mg kg-1 NAC, and (3) three episodes of IPC (10-min ischemia followed by 10-min reperfusion) prior to same ischemia. Hepatic reperfusion was maintained for a further 180 min, with hemodynamic and hepatic function parameters monitored throughout. Results: Plasma disappearance rate of indocyanine green and serum levels of aspartate transferase and alanine transferase showed no significant differences between groups. Although liver injury was obvious, reflected by hemodynamic, blood gas, and liver function tests, NAC and IPC failed to prevent decay in hepatic function in this canine model. Conclusion: The results do not support the hypothesis that short-term use of NAC and IPC is beneficial in hepatic surgery.

AB - Aims: We evaluated the possibility that repeated ischemic preconditioning or N-acetylcysteine (NAC) could prevent ischemia-reperfusion injury as determined by indocyanine green plasma disappearance rate (ICG-PDR) or has favorable hemodynamic effects during reperfusion in an in vivo canine liver model. Methods: Under general anesthesia, 3 groups of mongrel dogs (n = 5 per group) were subjected to (1) 60-min hepatic ischemia, (2) same ischemia preceded by intravenous administration of 150 mg kg-1 NAC, and (3) three episodes of IPC (10-min ischemia followed by 10-min reperfusion) prior to same ischemia. Hepatic reperfusion was maintained for a further 180 min, with hemodynamic and hepatic function parameters monitored throughout. Results: Plasma disappearance rate of indocyanine green and serum levels of aspartate transferase and alanine transferase showed no significant differences between groups. Although liver injury was obvious, reflected by hemodynamic, blood gas, and liver function tests, NAC and IPC failed to prevent decay in hepatic function in this canine model. Conclusion: The results do not support the hypothesis that short-term use of NAC and IPC is beneficial in hepatic surgery.

KW - Hemodynamics

KW - Ischemia-reperfusion injury

KW - N-acetylcysteine

KW - Preconditioning

UR - http://www.scopus.com/inward/record.url?scp=50249125354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50249125354&partnerID=8YFLogxK

U2 - 10.1159/000135707

DO - 10.1159/000135707

M3 - Article

C2 - 18520151

AN - SCOPUS:50249125354

VL - 41

SP - 226

EP - 230

JO - European Surgical Research

JF - European Surgical Research

SN - 0014-312X

IS - 2

ER -