Short-term effects of 1-nitropyrene on chromosomes and on oncogene/tumor suppressor gene expression in vivo

Zsuzsanna Pusztai, Andras Selypes, I. Ember

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In order to establish an animal model testing the effects of 1-nitropyrene in vivo at the oncogene level, we investigated the early biological effects of 1-nitropyrene in mice. The treatment of 6-8 week-old CBA/Ca mice with a single 30 μmol/kg body weight dose of 1-nitropyrene caused a significant increase in chromosome aberrations 48 hours after exposure. The aberrations were mainly of the euploid type. We also found elevated expression of the Ha-ras oncogene in the isolated total cellular RNA from the liver, lung, kidney, spleen and thymus. The highest increase was seen in the lung and it was also high in the spleen and in the thymus. There was a higher increase in the males than in the females in all organs. In this study we confirmed that early genetic alterations such as oncogene expression, can be examined not only in vitro, but also in vivo experiments.

Original languageEnglish
Pages (from-to)4489-4492
Number of pages4
JournalAnticancer Research
Volume18
Issue number6 A
Publication statusPublished - 1998

Fingerprint

1-nitropyrene
Tumor Suppressor Genes
Oncogenes
Chromosomes
Gene Expression
Thymus Gland
Spleen
Lung
Inbred CBA Mouse
ras Genes
Chromosome Aberrations
Animal Models
Body Weight
RNA
Kidney
Liver

Keywords

  • 1-Nitropyrene
  • c-myc
  • Chromosome-aberration
  • Gene-expression
  • Ha-ras
  • Mice
  • p53

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Short-term effects of 1-nitropyrene on chromosomes and on oncogene/tumor suppressor gene expression in vivo. / Pusztai, Zsuzsanna; Selypes, Andras; Ember, I.

In: Anticancer Research, Vol. 18, No. 6 A, 1998, p. 4489-4492.

Research output: Contribution to journalArticle

@article{f6bb21c0721f46ff9b7037314a450ea4,
title = "Short-term effects of 1-nitropyrene on chromosomes and on oncogene/tumor suppressor gene expression in vivo",
abstract = "In order to establish an animal model testing the effects of 1-nitropyrene in vivo at the oncogene level, we investigated the early biological effects of 1-nitropyrene in mice. The treatment of 6-8 week-old CBA/Ca mice with a single 30 μmol/kg body weight dose of 1-nitropyrene caused a significant increase in chromosome aberrations 48 hours after exposure. The aberrations were mainly of the euploid type. We also found elevated expression of the Ha-ras oncogene in the isolated total cellular RNA from the liver, lung, kidney, spleen and thymus. The highest increase was seen in the lung and it was also high in the spleen and in the thymus. There was a higher increase in the males than in the females in all organs. In this study we confirmed that early genetic alterations such as oncogene expression, can be examined not only in vitro, but also in vivo experiments.",
keywords = "1-Nitropyrene, c-myc, Chromosome-aberration, Gene-expression, Ha-ras, Mice, p53",
author = "Zsuzsanna Pusztai and Andras Selypes and I. Ember",
year = "1998",
language = "English",
volume = "18",
pages = "4489--4492",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "6 A",

}

TY - JOUR

T1 - Short-term effects of 1-nitropyrene on chromosomes and on oncogene/tumor suppressor gene expression in vivo

AU - Pusztai, Zsuzsanna

AU - Selypes, Andras

AU - Ember, I.

PY - 1998

Y1 - 1998

N2 - In order to establish an animal model testing the effects of 1-nitropyrene in vivo at the oncogene level, we investigated the early biological effects of 1-nitropyrene in mice. The treatment of 6-8 week-old CBA/Ca mice with a single 30 μmol/kg body weight dose of 1-nitropyrene caused a significant increase in chromosome aberrations 48 hours after exposure. The aberrations were mainly of the euploid type. We also found elevated expression of the Ha-ras oncogene in the isolated total cellular RNA from the liver, lung, kidney, spleen and thymus. The highest increase was seen in the lung and it was also high in the spleen and in the thymus. There was a higher increase in the males than in the females in all organs. In this study we confirmed that early genetic alterations such as oncogene expression, can be examined not only in vitro, but also in vivo experiments.

AB - In order to establish an animal model testing the effects of 1-nitropyrene in vivo at the oncogene level, we investigated the early biological effects of 1-nitropyrene in mice. The treatment of 6-8 week-old CBA/Ca mice with a single 30 μmol/kg body weight dose of 1-nitropyrene caused a significant increase in chromosome aberrations 48 hours after exposure. The aberrations were mainly of the euploid type. We also found elevated expression of the Ha-ras oncogene in the isolated total cellular RNA from the liver, lung, kidney, spleen and thymus. The highest increase was seen in the lung and it was also high in the spleen and in the thymus. There was a higher increase in the males than in the females in all organs. In this study we confirmed that early genetic alterations such as oncogene expression, can be examined not only in vitro, but also in vivo experiments.

KW - 1-Nitropyrene

KW - c-myc

KW - Chromosome-aberration

KW - Gene-expression

KW - Ha-ras

KW - Mice

KW - p53

UR - http://www.scopus.com/inward/record.url?scp=0032434932&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032434932&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 4489

EP - 4492

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 6 A

ER -