Short and long term biocompatibility of NeuroProbes silicon probes

László Grand, L. Wittner, Stanislav Herwik, Emmanuelle Göthelid, Patrick Ruther, Sven Oscarsson, Hercules Neves, Balázs Dombovári, Richárd Csercsa, G. Karmos, I. Ulbert

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Brain implants provide exceptional tools to understand and restore cerebral functions. The utility of these devices depends crucially on their biocompatibility and long term viability. We addressed these points by implanting non-functional, NeuroProbes silicon probes, without or with hyaluronic acid (Hya), dextran (Dex), dexamethasone (DexM), Hya. +. DexM coating, into rat neocortex. Light and transmission electron microscopy were used to investigate neuronal survival and glial response. The surface of explanted probes was examined in the scanning electron microscope. We show that blood vessel disruption during implantation could induce considerable tissue damage. If, however, probes could be inserted without major bleeding, light microscopical evidence of damage to surrounding neocortical tissue was much reduced. At distances less than 100 μm from the probe track a considerable neuron loss (∼40%) occurred at short survival times, while the neuronal numbers recovered close to control levels at longer survival. Slight gliosis was observed at both short and long term survivals. Electron microscopy showed neuronal cell bodies and synapses close (

Original languageEnglish
Pages (from-to)216-229
Number of pages14
JournalJournal of Neuroscience Methods
Volume189
Issue number2
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Silicon
Hyaluronic Acid
Dexamethasone
Light
Gliosis
Neocortex
Dextrans
Transmission Electron Microscopy
Neuroglia
Synapses
Blood Vessels
Electron Microscopy
Electrons
Hemorrhage
Neurons
Equipment and Supplies
Brain
Cell Body

Keywords

  • Glial reaction
  • Neuron density
  • Rat neocortex
  • Silicon probes
  • Surface treatment

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Short and long term biocompatibility of NeuroProbes silicon probes. / Grand, László; Wittner, L.; Herwik, Stanislav; Göthelid, Emmanuelle; Ruther, Patrick; Oscarsson, Sven; Neves, Hercules; Dombovári, Balázs; Csercsa, Richárd; Karmos, G.; Ulbert, I.

In: Journal of Neuroscience Methods, Vol. 189, No. 2, 06.2010, p. 216-229.

Research output: Contribution to journalArticle

Grand, L, Wittner, L, Herwik, S, Göthelid, E, Ruther, P, Oscarsson, S, Neves, H, Dombovári, B, Csercsa, R, Karmos, G & Ulbert, I 2010, 'Short and long term biocompatibility of NeuroProbes silicon probes', Journal of Neuroscience Methods, vol. 189, no. 2, pp. 216-229. https://doi.org/10.1016/j.jneumeth.2010.04.009
Grand, László ; Wittner, L. ; Herwik, Stanislav ; Göthelid, Emmanuelle ; Ruther, Patrick ; Oscarsson, Sven ; Neves, Hercules ; Dombovári, Balázs ; Csercsa, Richárd ; Karmos, G. ; Ulbert, I. / Short and long term biocompatibility of NeuroProbes silicon probes. In: Journal of Neuroscience Methods. 2010 ; Vol. 189, No. 2. pp. 216-229.
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