Shifting the paradigm

The putative mitochondrial protein ABCB6 resides in the lysosomes of cells and in the plasma membrane of erythrocytes

Katalin Kiss, Anna Brozik, Nora Kucsma, Alexandra Toth, Melinda Gera, Laurence Berry, Alice Vallentin, Henri Vial, Michel Vidal, G. Szakács

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

ABCB6, a member of the adenosine triphosphate-binding cassette (ABC) transporter family, has been proposed to be responsible for the mitochondrial uptake of porphyrins. Here we show that ABCB6 is a glycoprotein present in the membrane of mature erythrocytes and in exosomes released from reticulocytes during the final steps of erythroid maturation. Consistent with its presence in exosomes, endogenous ABCB6 is localized to the endo/lysosomal compartment, and is absent from the mitochondria of cells. Knock-down studies demonstrate that ABCB6 function is not required for de novo heme biosynthesis in differentiating K562 cells, excluding this ABC transporter as a key regulator of porphyrin synthesis. We confirm the mitochondrial localization of ABCB7, ABCB8 and ABCB10, suggesting that only three ABC transporters should be classified as mitochondrial proteins. Taken together, our results challenge the current paradigm linking the expression and function of ABCB6 to mitochondria.

Original languageEnglish
Article numbere37378
JournalPLoS One
Volume7
Issue number5
DOIs
Publication statusPublished - May 24 2012

Fingerprint

adenosine triphosphate
Mitochondrial Proteins
lysosomes
Cell membranes
Lysosomes
exosomes
Exosomes
transporters
Mitochondria
porphyrins
erythrocytes
plasma membrane
Adenosine Triphosphate
Erythrocytes
Porphyrins
Cell Membrane
mitochondria
membrane glycoproteins
reticulocytes
K562 Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Shifting the paradigm : The putative mitochondrial protein ABCB6 resides in the lysosomes of cells and in the plasma membrane of erythrocytes. / Kiss, Katalin; Brozik, Anna; Kucsma, Nora; Toth, Alexandra; Gera, Melinda; Berry, Laurence; Vallentin, Alice; Vial, Henri; Vidal, Michel; Szakács, G.

In: PLoS One, Vol. 7, No. 5, e37378, 24.05.2012.

Research output: Contribution to journalArticle

Kiss, Katalin ; Brozik, Anna ; Kucsma, Nora ; Toth, Alexandra ; Gera, Melinda ; Berry, Laurence ; Vallentin, Alice ; Vial, Henri ; Vidal, Michel ; Szakács, G. / Shifting the paradigm : The putative mitochondrial protein ABCB6 resides in the lysosomes of cells and in the plasma membrane of erythrocytes. In: PLoS One. 2012 ; Vol. 7, No. 5.
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