Sexual reproduction as the cause of heat resistance in the food spoilage fungus Byssochlamys spectabilis (anamorph Paecilomyces variotii)

Jos Houbraken, J. Varga, Emilia Rico-Munoz, Shawn Johnson, Robert A. Samson

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Paecilomyces variotii is a common cosmopolitan species that is able to spoil various food- and feedstuffs and is frequently encountered in heat-treated products. However, isolates from heat-treated products rarely form ascospores. In this study we examined by using molecular techniques and mating tests whether this species can undergo a sexual cycle and form ascospores. The population structure of this species was examined by analyzing the nuclear ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and the 5.8S rRNA gene, as well as partial β-tubulin, actin, and calmodulin gene sequences. Phylogenetic analyses revealed that P. variotii is a highly variable species. Partition homogeneity tests revealed that P. variotii has a recombining population structure. In addition to sequence analyses, mating experiments indicated that P. variotii is able to form ascomata and ascospores in culture in a heterothallic manner. The distribution of MAT1-1 and MAT1-2 genes showed a 1:1 ratio in the progeny of the mating experiments. From the sequence analyses and mating data we conclude that P. variotii is the anamorph of Talaromyces spectabilis and that it has a biallelic heterothallic mating system. Since Paecilomyces sensu stricto anamorphs group within Byssochlamys, a new combination Byssochlamys spectabilis is proposed.

Original languageEnglish
Pages (from-to)1613-1619
Number of pages7
JournalApplied and Environmental Microbiology
Volume74
Issue number5
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Byssochlamys
Paecilomyces
food spoilage
anamorphs
sexual reproduction
heat tolerance
Reproduction
Sequence Analysis
Talaromyces
Fungi
Hot Temperature
fungus
Food
fungi
food
Calmodulin
Tubulin
ascospores
rRNA Genes
Population

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Sexual reproduction as the cause of heat resistance in the food spoilage fungus Byssochlamys spectabilis (anamorph Paecilomyces variotii). / Houbraken, Jos; Varga, J.; Rico-Munoz, Emilia; Johnson, Shawn; Samson, Robert A.

In: Applied and Environmental Microbiology, Vol. 74, No. 5, 03.2008, p. 1613-1619.

Research output: Contribution to journalArticle

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