Sexual dimorphism in renal ischemia-reperfusion injury in rats: Possible role of endothelin

V. Müller, G. Losonczy, Uwe Heemann, A. Vannay, Andrea Fekete, G. Reusz, T. Tulassay, Attila J. Szabó

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Background. Postischemic organ dysfunction is influenced by gender and sexual steroids. Methods. To compare the susceptibility of the kidney to postischemic failure between sexes, the left vascular pedicle was clamped for 50 minutes in anesthetized male and female Wistar rats. Survival rate, renal and systemic hemodynamics and renal prepro-endothelin (pp-ET) mRNA expression were measured. Results. Eight percent of males as compared to 75% of females survived for more than 7 days. Previous orchidectomy of mature rats or sexual immaturity improved the rate of 7 day survival to 67% and 58%, respectively, as compared to intact males (P <0.05). Estradiol treatment of mature male animals also resulted in a significantly better survival. Ovariectomy, sexual immaturity or testosterone treatment had no impact on the course of renal failure in females. The early postischemic recovery of renal blood flow was delayed due to a dramatic increase in renal vascular resistance in male versus female rats. The expression of pp-ET gene in the kidneys was increased at 5 minutes following reperfusion and was significantly higher 2 hours after ischemia in males, but not in females. Pretreatment with the endothelin A receptor antagonist LU 135252 provided indistinguishable survival rates in intact male and female rats after warm renal ischemia. Conclusion. Female rats enjoy relative protection against postischemic renal failure. Furthermore, in intact males the effects of androgens upon ischemic kidney damage seem to be mediated by endothelin-induced vascular changes.

Original languageEnglish
Pages (from-to)1364-1371
Number of pages8
JournalKidney International
Volume62
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Endothelins
Reperfusion Injury
Sex Characteristics
Kidney
Renal Insufficiency
Blood Vessels
Warm Ischemia
Orchiectomy
Renal Circulation
Ovariectomy
Vascular Resistance
Androgens
Reperfusion
Testosterone
Wistar Rats
Estradiol
Ischemia
Hemodynamics
Steroids
Messenger RNA

Keywords

  • Acute renal failure
  • Endothelin
  • Hemodynamics
  • Postischemic kidney failure
  • Renal blood flow
  • Transplantation
  • Vascular resistance
  • Warm ischemia

ASJC Scopus subject areas

  • Nephrology

Cite this

Sexual dimorphism in renal ischemia-reperfusion injury in rats : Possible role of endothelin. / Müller, V.; Losonczy, G.; Heemann, Uwe; Vannay, A.; Fekete, Andrea; Reusz, G.; Tulassay, T.; Szabó, Attila J.

In: Kidney International, Vol. 62, No. 4, 2002, p. 1364-1371.

Research output: Contribution to journalArticle

@article{79c4a227773e4fcbadc9d29e3746404f,
title = "Sexual dimorphism in renal ischemia-reperfusion injury in rats: Possible role of endothelin",
abstract = "Background. Postischemic organ dysfunction is influenced by gender and sexual steroids. Methods. To compare the susceptibility of the kidney to postischemic failure between sexes, the left vascular pedicle was clamped for 50 minutes in anesthetized male and female Wistar rats. Survival rate, renal and systemic hemodynamics and renal prepro-endothelin (pp-ET) mRNA expression were measured. Results. Eight percent of males as compared to 75{\%} of females survived for more than 7 days. Previous orchidectomy of mature rats or sexual immaturity improved the rate of 7 day survival to 67{\%} and 58{\%}, respectively, as compared to intact males (P <0.05). Estradiol treatment of mature male animals also resulted in a significantly better survival. Ovariectomy, sexual immaturity or testosterone treatment had no impact on the course of renal failure in females. The early postischemic recovery of renal blood flow was delayed due to a dramatic increase in renal vascular resistance in male versus female rats. The expression of pp-ET gene in the kidneys was increased at 5 minutes following reperfusion and was significantly higher 2 hours after ischemia in males, but not in females. Pretreatment with the endothelin A receptor antagonist LU 135252 provided indistinguishable survival rates in intact male and female rats after warm renal ischemia. Conclusion. Female rats enjoy relative protection against postischemic renal failure. Furthermore, in intact males the effects of androgens upon ischemic kidney damage seem to be mediated by endothelin-induced vascular changes.",
keywords = "Acute renal failure, Endothelin, Hemodynamics, Postischemic kidney failure, Renal blood flow, Transplantation, Vascular resistance, Warm ischemia",
author = "V. M{\"u}ller and G. Losonczy and Uwe Heemann and A. Vannay and Andrea Fekete and G. Reusz and T. Tulassay and Szab{\'o}, {Attila J.}",
year = "2002",
doi = "10.1046/j.1523-1755.2002.00590.x",
language = "English",
volume = "62",
pages = "1364--1371",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Sexual dimorphism in renal ischemia-reperfusion injury in rats

T2 - Possible role of endothelin

AU - Müller, V.

AU - Losonczy, G.

AU - Heemann, Uwe

AU - Vannay, A.

AU - Fekete, Andrea

AU - Reusz, G.

AU - Tulassay, T.

AU - Szabó, Attila J.

PY - 2002

Y1 - 2002

N2 - Background. Postischemic organ dysfunction is influenced by gender and sexual steroids. Methods. To compare the susceptibility of the kidney to postischemic failure between sexes, the left vascular pedicle was clamped for 50 minutes in anesthetized male and female Wistar rats. Survival rate, renal and systemic hemodynamics and renal prepro-endothelin (pp-ET) mRNA expression were measured. Results. Eight percent of males as compared to 75% of females survived for more than 7 days. Previous orchidectomy of mature rats or sexual immaturity improved the rate of 7 day survival to 67% and 58%, respectively, as compared to intact males (P <0.05). Estradiol treatment of mature male animals also resulted in a significantly better survival. Ovariectomy, sexual immaturity or testosterone treatment had no impact on the course of renal failure in females. The early postischemic recovery of renal blood flow was delayed due to a dramatic increase in renal vascular resistance in male versus female rats. The expression of pp-ET gene in the kidneys was increased at 5 minutes following reperfusion and was significantly higher 2 hours after ischemia in males, but not in females. Pretreatment with the endothelin A receptor antagonist LU 135252 provided indistinguishable survival rates in intact male and female rats after warm renal ischemia. Conclusion. Female rats enjoy relative protection against postischemic renal failure. Furthermore, in intact males the effects of androgens upon ischemic kidney damage seem to be mediated by endothelin-induced vascular changes.

AB - Background. Postischemic organ dysfunction is influenced by gender and sexual steroids. Methods. To compare the susceptibility of the kidney to postischemic failure between sexes, the left vascular pedicle was clamped for 50 minutes in anesthetized male and female Wistar rats. Survival rate, renal and systemic hemodynamics and renal prepro-endothelin (pp-ET) mRNA expression were measured. Results. Eight percent of males as compared to 75% of females survived for more than 7 days. Previous orchidectomy of mature rats or sexual immaturity improved the rate of 7 day survival to 67% and 58%, respectively, as compared to intact males (P <0.05). Estradiol treatment of mature male animals also resulted in a significantly better survival. Ovariectomy, sexual immaturity or testosterone treatment had no impact on the course of renal failure in females. The early postischemic recovery of renal blood flow was delayed due to a dramatic increase in renal vascular resistance in male versus female rats. The expression of pp-ET gene in the kidneys was increased at 5 minutes following reperfusion and was significantly higher 2 hours after ischemia in males, but not in females. Pretreatment with the endothelin A receptor antagonist LU 135252 provided indistinguishable survival rates in intact male and female rats after warm renal ischemia. Conclusion. Female rats enjoy relative protection against postischemic renal failure. Furthermore, in intact males the effects of androgens upon ischemic kidney damage seem to be mediated by endothelin-induced vascular changes.

KW - Acute renal failure

KW - Endothelin

KW - Hemodynamics

KW - Postischemic kidney failure

KW - Renal blood flow

KW - Transplantation

KW - Vascular resistance

KW - Warm ischemia

UR - http://www.scopus.com/inward/record.url?scp=0036380927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036380927&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1755.2002.00590.x

DO - 10.1046/j.1523-1755.2002.00590.x

M3 - Article

C2 - 12234307

AN - SCOPUS:0036380927

VL - 62

SP - 1364

EP - 1371

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 4

ER -