Sex-specific chronic stress response at the level of adrenal gland modified sexual hormone and leptin receptors

Marta Balog, Milan Miljanović, Senka Blažetić, Irena Labak, Vedrana Ivić, Barbara Viljetić, A. Borbély, Z. Papp, Robert Blažeković, Sandor G. Vari, Miklós Fagyas, Marija Heffer

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Abstract

Aim: To compare cardiometabolic risk-related biochemical markers and sexual hormone and leptin receptors in the adrenal gland of rat males, non-ovariectomized females (NON-OVX), and ovariectomized females (OVX) under chronic stress. Methods: Forty six 16-week-old Sprague-Dawley rats were divided into male, NON-OVX, and OVX group and exposed to chronic stress or kept as controls. Weight, glucose tolerance test (GTT), serum concentration of glucose, and cholesterol were measured. Adrenal glands were collected at the age of 28 weeks and immunohistochemical staining against estrogen beta (ERβ), progesterone (PR), testosterone (AR), and leptin (Ob-R) receptors was performed. Results: Body weight, GTT, serum cholesterol, and glucose changed in response to stress as expected and validated the applied stress protocol. Stressed males had significantly higher number of ERβ receptors in comparison to control group (P = 0.028). Stressed NON-OVX group had significantly decreased AR in comparison to control group (P = 0.007). The levels of PR did not change in any consistent pattern. The levels of Ob-R increased upon stress in all groups, but the significant difference was reached only in the case of stressed OVX group compared to control (P = 0.033). Conclusion: Chronic stress response was sex specific. OVX females had similar biochemical parameters as males. Changes upon chronic stress in adrenal gland were related to an increase in testosterone receptor in females and decrease in estrogen receptor in males.

Original languageEnglish
Pages (from-to)104-113
Number of pages10
JournalCroatian Medical Journal
Volume56
Issue number2
DOIs
Publication statusPublished - 2015

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Leptin Receptors
Adrenal Glands
Hormones
Glucose Tolerance Test
Progesterone
Cholesterol
Glucose
Estrogen Receptor beta
Control Groups
Androgen Receptors
Leptin
Serum
Estrogen Receptors
Sprague Dawley Rats
Testosterone
Estrogens
Biomarkers
Body Weight
Staining and Labeling
Weights and Measures

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Balog, M., Miljanović, M., Blažetić, S., Labak, I., Ivić, V., Viljetić, B., ... Heffer, M. (2015). Sex-specific chronic stress response at the level of adrenal gland modified sexual hormone and leptin receptors. Croatian Medical Journal, 56(2), 104-113. https://doi.org/10.3325/cmj.2015.56.104

Sex-specific chronic stress response at the level of adrenal gland modified sexual hormone and leptin receptors. / Balog, Marta; Miljanović, Milan; Blažetić, Senka; Labak, Irena; Ivić, Vedrana; Viljetić, Barbara; Borbély, A.; Papp, Z.; Blažeković, Robert; Vari, Sandor G.; Fagyas, Miklós; Heffer, Marija.

In: Croatian Medical Journal, Vol. 56, No. 2, 2015, p. 104-113.

Research output: Contribution to journalArticle

Balog, M, Miljanović, M, Blažetić, S, Labak, I, Ivić, V, Viljetić, B, Borbély, A, Papp, Z, Blažeković, R, Vari, SG, Fagyas, M & Heffer, M 2015, 'Sex-specific chronic stress response at the level of adrenal gland modified sexual hormone and leptin receptors', Croatian Medical Journal, vol. 56, no. 2, pp. 104-113. https://doi.org/10.3325/cmj.2015.56.104
Balog, Marta ; Miljanović, Milan ; Blažetić, Senka ; Labak, Irena ; Ivić, Vedrana ; Viljetić, Barbara ; Borbély, A. ; Papp, Z. ; Blažeković, Robert ; Vari, Sandor G. ; Fagyas, Miklós ; Heffer, Marija. / Sex-specific chronic stress response at the level of adrenal gland modified sexual hormone and leptin receptors. In: Croatian Medical Journal. 2015 ; Vol. 56, No. 2. pp. 104-113.
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AU - Viljetić, Barbara

AU - Borbély, A.

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