Sex determination using circulating cell-free fetal DNA in small volume of maternal plasma in elephants

Boglárka Vincze, András Gáspárdy, Alexandra Biácsi, Endre Ákos Papp, László Garamvölgyi, Endre Sós, Sándor Cseh, Gábor Kovács, Zsolt Pádár, Petra Zenke

Research output: Contribution to journalArticle

Abstract

The genetic sexing of animals having long gestation periods offers significant benefits in regard to breeding management among their populations living in captivity. In our study, a new increased-sensitivity PCR method for fetal sexing was developed and tested successfully on elephants, from only a small volume of maternal plasma. Suitable sensitivity was obtained by using short, reduced amplicon lengths with fluorescent labelling for capillary electrophoresis detection. The fundamental principle for this technique was based on the detection of two Y-specific markers (AmelY and SRY), the presence of which indicates the mother is carrying a male fetus and the absence of these markers designates a female fetus. As a reaction control, the X-chromosomal marker (PlpX) was used. To the best of our knowledge, this is the first report on this topic, confirming the presence of fetal cell-free DNA from the plasma of a pregnant captive elephant, and demonstrating a new opportunity for non-invasive assessment in fetal sex determination.

Original languageEnglish
Article number15254
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Plasma Volume
Fetus
Mothers
DNA
Capillary Electrophoresis
Breeding
Pregnancy
Polymerase Chain Reaction
Population

ASJC Scopus subject areas

  • General

Cite this

Vincze, B., Gáspárdy, A., Biácsi, A., Papp, E. Á., Garamvölgyi, L., Sós, E., ... Zenke, P. (2019). Sex determination using circulating cell-free fetal DNA in small volume of maternal plasma in elephants. Scientific reports, 9(1), [15254]. https://doi.org/10.1038/s41598-019-51641-8

Sex determination using circulating cell-free fetal DNA in small volume of maternal plasma in elephants. / Vincze, Boglárka; Gáspárdy, András; Biácsi, Alexandra; Papp, Endre Ákos; Garamvölgyi, László; Sós, Endre; Cseh, Sándor; Kovács, Gábor; Pádár, Zsolt; Zenke, Petra.

In: Scientific reports, Vol. 9, No. 1, 15254, 01.12.2019.

Research output: Contribution to journalArticle

Vincze, B, Gáspárdy, A, Biácsi, A, Papp, EÁ, Garamvölgyi, L, Sós, E, Cseh, S, Kovács, G, Pádár, Z & Zenke, P 2019, 'Sex determination using circulating cell-free fetal DNA in small volume of maternal plasma in elephants', Scientific reports, vol. 9, no. 1, 15254. https://doi.org/10.1038/s41598-019-51641-8
Vincze, Boglárka ; Gáspárdy, András ; Biácsi, Alexandra ; Papp, Endre Ákos ; Garamvölgyi, László ; Sós, Endre ; Cseh, Sándor ; Kovács, Gábor ; Pádár, Zsolt ; Zenke, Petra. / Sex determination using circulating cell-free fetal DNA in small volume of maternal plasma in elephants. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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