Sex chromosome changes after sex-mismatched allogeneic bone marrow transplantation can mislead the chimerism analysis

Donát Alpár, Gergely Nagy, Carsten Hohoff, Béla Kajtár, Katalin Bartyik, Judit Hermesz, Pál Jáksó, H. Andrikovics, L. Kereskai, László Pajor

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A 12-year-old male with pre-B-cell acute lymphoblastic leukemia with cryptic BCR/ABL rearrangement underwent sex-mismatched allogeneic bone marrow transplantation (allo-BMT). Contradictory results were provided by various chimerism analyses 3 months later. Y-chromosome-specific quantitative polymerase chain reaction and sex chromosome-specific interphase fluorescence in situ hybridization (i-FISH) showed complete donor chimerism. Analysis of autosomal short tandem repeats (A-STR), BCR/ABL i-FISH test, and X-STR haplotype indicated relapse. Metaphase-FISH and combined BCR/ABL and sex chromosome-specific i-FISH patterns revealed loss of the Y-chromosome and duplication of the X-chromosome in the host cells. Sex chromosome changes after allo-BMT can cause significant difficulties in chimerism analysis.

Original languageEnglish
Pages (from-to)1239-1242
Number of pages4
JournalPediatric Blood and Cancer
Volume55
Issue number6
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Chimerism
Sex Chromosomes
Interphase
Homologous Transplantation
Fluorescence In Situ Hybridization
Bone Marrow Transplantation
Y Chromosome
Chromosome Duplication
B-Lymphoid Precursor Cells
X Chromosome
Metaphase
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Microsatellite Repeats
Haplotypes
Recurrence
Polymerase Chain Reaction

Keywords

  • Allogeneic bone marrow transplantation
  • Chimerism
  • FISH
  • Sex chromosomes
  • STR
  • Y-QPCR

ASJC Scopus subject areas

  • Oncology
  • Pediatrics, Perinatology, and Child Health
  • Hematology

Cite this

Sex chromosome changes after sex-mismatched allogeneic bone marrow transplantation can mislead the chimerism analysis. / Alpár, Donát; Nagy, Gergely; Hohoff, Carsten; Kajtár, Béla; Bartyik, Katalin; Hermesz, Judit; Jáksó, Pál; Andrikovics, H.; Kereskai, L.; Pajor, László.

In: Pediatric Blood and Cancer, Vol. 55, No. 6, 12.2010, p. 1239-1242.

Research output: Contribution to journalArticle

Alpár, Donát ; Nagy, Gergely ; Hohoff, Carsten ; Kajtár, Béla ; Bartyik, Katalin ; Hermesz, Judit ; Jáksó, Pál ; Andrikovics, H. ; Kereskai, L. ; Pajor, László. / Sex chromosome changes after sex-mismatched allogeneic bone marrow transplantation can mislead the chimerism analysis. In: Pediatric Blood and Cancer. 2010 ; Vol. 55, No. 6. pp. 1239-1242.
@article{79c9a2555e0643c39871f9fb579d5bb5,
title = "Sex chromosome changes after sex-mismatched allogeneic bone marrow transplantation can mislead the chimerism analysis",
abstract = "A 12-year-old male with pre-B-cell acute lymphoblastic leukemia with cryptic BCR/ABL rearrangement underwent sex-mismatched allogeneic bone marrow transplantation (allo-BMT). Contradictory results were provided by various chimerism analyses 3 months later. Y-chromosome-specific quantitative polymerase chain reaction and sex chromosome-specific interphase fluorescence in situ hybridization (i-FISH) showed complete donor chimerism. Analysis of autosomal short tandem repeats (A-STR), BCR/ABL i-FISH test, and X-STR haplotype indicated relapse. Metaphase-FISH and combined BCR/ABL and sex chromosome-specific i-FISH patterns revealed loss of the Y-chromosome and duplication of the X-chromosome in the host cells. Sex chromosome changes after allo-BMT can cause significant difficulties in chimerism analysis.",
keywords = "Allogeneic bone marrow transplantation, Chimerism, FISH, Sex chromosomes, STR, Y-QPCR",
author = "Don{\'a}t Alp{\'a}r and Gergely Nagy and Carsten Hohoff and B{\'e}la Kajt{\'a}r and Katalin Bartyik and Judit Hermesz and P{\'a}l J{\'a}ks{\'o} and H. Andrikovics and L. Kereskai and L{\'a}szl{\'o} Pajor",
year = "2010",
month = "12",
doi = "10.1002/pbc.22617",
language = "English",
volume = "55",
pages = "1239--1242",
journal = "Pediatric Blood and Cancer",
issn = "1545-5009",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Sex chromosome changes after sex-mismatched allogeneic bone marrow transplantation can mislead the chimerism analysis

AU - Alpár, Donát

AU - Nagy, Gergely

AU - Hohoff, Carsten

AU - Kajtár, Béla

AU - Bartyik, Katalin

AU - Hermesz, Judit

AU - Jáksó, Pál

AU - Andrikovics, H.

AU - Kereskai, L.

AU - Pajor, László

PY - 2010/12

Y1 - 2010/12

N2 - A 12-year-old male with pre-B-cell acute lymphoblastic leukemia with cryptic BCR/ABL rearrangement underwent sex-mismatched allogeneic bone marrow transplantation (allo-BMT). Contradictory results were provided by various chimerism analyses 3 months later. Y-chromosome-specific quantitative polymerase chain reaction and sex chromosome-specific interphase fluorescence in situ hybridization (i-FISH) showed complete donor chimerism. Analysis of autosomal short tandem repeats (A-STR), BCR/ABL i-FISH test, and X-STR haplotype indicated relapse. Metaphase-FISH and combined BCR/ABL and sex chromosome-specific i-FISH patterns revealed loss of the Y-chromosome and duplication of the X-chromosome in the host cells. Sex chromosome changes after allo-BMT can cause significant difficulties in chimerism analysis.

AB - A 12-year-old male with pre-B-cell acute lymphoblastic leukemia with cryptic BCR/ABL rearrangement underwent sex-mismatched allogeneic bone marrow transplantation (allo-BMT). Contradictory results were provided by various chimerism analyses 3 months later. Y-chromosome-specific quantitative polymerase chain reaction and sex chromosome-specific interphase fluorescence in situ hybridization (i-FISH) showed complete donor chimerism. Analysis of autosomal short tandem repeats (A-STR), BCR/ABL i-FISH test, and X-STR haplotype indicated relapse. Metaphase-FISH and combined BCR/ABL and sex chromosome-specific i-FISH patterns revealed loss of the Y-chromosome and duplication of the X-chromosome in the host cells. Sex chromosome changes after allo-BMT can cause significant difficulties in chimerism analysis.

KW - Allogeneic bone marrow transplantation

KW - Chimerism

KW - FISH

KW - Sex chromosomes

KW - STR

KW - Y-QPCR

UR - http://www.scopus.com/inward/record.url?scp=77958530267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958530267&partnerID=8YFLogxK

U2 - 10.1002/pbc.22617

DO - 10.1002/pbc.22617

M3 - Article

VL - 55

SP - 1239

EP - 1242

JO - Pediatric Blood and Cancer

JF - Pediatric Blood and Cancer

SN - 1545-5009

IS - 6

ER -