Sex chromosome changes after sex-mismatched allogeneic bone marrow transplantation can mislead the chimerism analysis

Donát Alpár, Gergely Nagy, Carsten Hohoff, Béla Kajtár, Katalin Bartyik, Judit Hermesz, Pál Jáksó, Hajnalka Andrikovics, László Kereskai, László Pajor

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4 Citations (Scopus)

Abstract

A 12-year-old male with pre-B-cell acute lymphoblastic leukemia with cryptic BCR/ABL rearrangement underwent sex-mismatched allogeneic bone marrow transplantation (allo-BMT). Contradictory results were provided by various chimerism analyses 3 months later. Y-chromosome-specific quantitative polymerase chain reaction and sex chromosome-specific interphase fluorescence in situ hybridization (i-FISH) showed complete donor chimerism. Analysis of autosomal short tandem repeats (A-STR), BCR/ABL i-FISH test, and X-STR haplotype indicated relapse. Metaphase-FISH and combined BCR/ABL and sex chromosome-specific i-FISH patterns revealed loss of the Y-chromosome and duplication of the X-chromosome in the host cells. Sex chromosome changes after allo-BMT can cause significant difficulties in chimerism analysis.

Original languageEnglish
Pages (from-to)1239-1242
Number of pages4
JournalPediatric Blood and Cancer
Volume55
Issue number6
DOIs
Publication statusPublished - Dec 1 2010

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Keywords

  • Allogeneic bone marrow transplantation
  • Chimerism
  • FISH
  • STR
  • Sex chromosomes
  • Y-QPCR

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Hematology
  • Oncology

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