Seven in absentia proteins affect plant growth and nodulation in Medicago truncatula

Griet Den Herder, Annick De Keyser, Riet De Rycke, Stephane Rombauts, Willem Van De Velde, María R. Clemente, Christa Verplancke, Peter Mergaert, É. Kondorosi, Marcelle Holster, Sofie Goormachtig

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Protein ubiquitination is a posttranslational regulatory process essential for plant growth and interaction with the environment. E3 ligases, to which the seven in absentia (SINA) proteins belong, determine the specificity by selecting the target proteins for ubiquitination. SINA proteins are found in animals as well as in plants, and a small gene family with highly related members has been identified in the genome of rice (Oryza sativa), Arabidopsis (Arabidopsis thaliana), Medicago truncatula, and poplar (Populus trichocarpa). To acquire insight into the function of SINA proteins in nodulation, a dominant negative form of the Arabidopsis SINAT5 was ectopically expressed in the model legume M. truncatula. After rhizobial inoculation of the 35S:SINAT5DN transgenic plants, fewer nodules were formed than in control plants, and most nodules remained small and white, a sign of impaired symbiosis. Defects in rhizobial infection and symbiosome formation were observed by extensive microscopic analysis. Besides the nodulation phenotype, transgenic plants were affected in shoot growth, leaf size, and lateral root number. This work illustrates a function for SINA E3 ligases in a broad spectrum of plant developmental processes, including nodulation.

Original languageEnglish
Pages (from-to)369-382
Number of pages14
JournalPlant Physiology
Volume148
Issue number1
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Medicago truncatula
nodulation
Arabidopsis
plant growth
Ubiquitin-Protein Ligases
Ubiquitination
Genetically Modified Plants
Growth
ligases
proteins
transgenic plants
Populus
Symbiosis
Populus balsamifera subsp. trichocarpa
Fabaceae
Proteins
symbiosis
Genome
Oryza sativa
Phenotype

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Herder, G. D., De Keyser, A., De Rycke, R., Rombauts, S., Van De Velde, W., Clemente, M. R., ... Goormachtig, S. (2008). Seven in absentia proteins affect plant growth and nodulation in Medicago truncatula. Plant Physiology, 148(1), 369-382. https://doi.org/10.1104/pp.108.119453

Seven in absentia proteins affect plant growth and nodulation in Medicago truncatula. / Herder, Griet Den; De Keyser, Annick; De Rycke, Riet; Rombauts, Stephane; Van De Velde, Willem; Clemente, María R.; Verplancke, Christa; Mergaert, Peter; Kondorosi, É.; Holster, Marcelle; Goormachtig, Sofie.

In: Plant Physiology, Vol. 148, No. 1, 09.2008, p. 369-382.

Research output: Contribution to journalArticle

Herder, GD, De Keyser, A, De Rycke, R, Rombauts, S, Van De Velde, W, Clemente, MR, Verplancke, C, Mergaert, P, Kondorosi, É, Holster, M & Goormachtig, S 2008, 'Seven in absentia proteins affect plant growth and nodulation in Medicago truncatula', Plant Physiology, vol. 148, no. 1, pp. 369-382. https://doi.org/10.1104/pp.108.119453
Herder GD, De Keyser A, De Rycke R, Rombauts S, Van De Velde W, Clemente MR et al. Seven in absentia proteins affect plant growth and nodulation in Medicago truncatula. Plant Physiology. 2008 Sep;148(1):369-382. https://doi.org/10.1104/pp.108.119453
Herder, Griet Den ; De Keyser, Annick ; De Rycke, Riet ; Rombauts, Stephane ; Van De Velde, Willem ; Clemente, María R. ; Verplancke, Christa ; Mergaert, Peter ; Kondorosi, É. ; Holster, Marcelle ; Goormachtig, Sofie. / Seven in absentia proteins affect plant growth and nodulation in Medicago truncatula. In: Plant Physiology. 2008 ; Vol. 148, No. 1. pp. 369-382.
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