Serum thyroglobulin antibody levels within or near to the reference range may interfere with thyroglobulin measurement

Zoltán Löcsei, I. Szabolcs, K. Rácz, G. Kovács, Dóra Horváth, Erzsébet Toldy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

High concentration of thyroglobulin antibodies (TgAb) is a major limiting factor of thyroglobulin measurements in patients with differentiated thyroid cancer. We investigated whether thyroglobulin antibody added to serum samples could interfere with the thyroglobulin assay. Thyroglobulin levels in serum samples with different concentrations of thyroglobulin were measured by electrochemiluminescence immunoassay before and after the addition of increasing concentrations of thyroglobulin antibody using the secondary calibrator solution of the thyroglobulin assay kit containing sheep thyroglobulin antibody to reach thyroglobulin antibody levels within or near to the reference range. Thyroglobulin and thyroglobulin antibody concentrations were also measured in 134 serum samples from 27 patients after thyroid ablation. There was a strong negative association (slope = -1.179) between thyroglobulin antibody and thyroglobulin concentrations in samples with added thyroglobulin antibody (beta = -0.86; P <0.001). Changes in thyroglobulin concentrations were described mathematically as loss of thyroglobulin% = -0.2408 x Ln(thyroglobulin antibody IU/ml) + 0.1944. Thyroglobulin concentrations were significantly lower than those calculated from experiments with added thyroglobulin antibody in 26/134 samples from patients after thyroid ablation. We conclude that if the same TgAb interference exists in the presence of naturally occurring human TgAb, our observation may prove to be useful during follow-up of patients with differentiated thyroid cancer. However, further studies are needed to explore the clinical relevance of thyroglobulin antibody levels within or near to the reference range in monitoring these patients.

Original languageEnglish
Pages (from-to)365-370
Number of pages6
JournalBiochemia Medica
Volume22
Issue number3
Publication statusPublished - 2012

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Thyroglobulin
Reference Values
Antibodies
Serum
Ablation
Thyroid Neoplasms
Assays
Thyroid Gland

Keywords

  • Antibody interference
  • Assay
  • Thyroglobulin
  • Thyroglobulin antibodies

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Serum thyroglobulin antibody levels within or near to the reference range may interfere with thyroglobulin measurement. / Löcsei, Zoltán; Szabolcs, I.; Rácz, K.; Kovács, G.; Horváth, Dóra; Toldy, Erzsébet.

In: Biochemia Medica, Vol. 22, No. 3, 2012, p. 365-370.

Research output: Contribution to journalArticle

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