Serum N-glycosylation in Parkinson’s disease

A novel approach for potential alterations

Csaba Váradi, Károly Nehéz, Olivér Hornyák, B. Viskolcz, Jonathan Bones

Research output: Contribution to journalArticle

Abstract

In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson’s disease. Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte. The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing. The annotated structures were quantified in 15 controls and 15 Parkinson’s disease patients by label-free quantitation. Lower sialylation and increased fucosylation were found in Parkinson’s disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids. The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.

Original languageEnglish
Article number2220
JournalMolecules
Volume24
Issue number12
DOIs
Publication statusPublished - Jun 13 2019

Fingerprint

Parkinson disease
Glycosylation
serums
Polysaccharides
Parkinson Disease
Capillary electrophoresis
Capillary Electrophoresis
Mass spectrometry
Support vector machines
Labels
electrophoresis
Serum
Sialic Acids
elimination
Coatings
mass spectroscopy
Glycoside Hydrolases
Tandem Mass Spectrometry
coatings
Spectrometry

Keywords

  • Capillary electrophoresis
  • Glycosylation
  • Label-free quantitation
  • Parkinson’s disease
  • Support vector machine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Serum N-glycosylation in Parkinson’s disease : A novel approach for potential alterations. / Váradi, Csaba; Nehéz, Károly; Hornyák, Olivér; Viskolcz, B.; Bones, Jonathan.

In: Molecules, Vol. 24, No. 12, 2220, 13.06.2019.

Research output: Contribution to journalArticle

Váradi, Csaba ; Nehéz, Károly ; Hornyák, Olivér ; Viskolcz, B. ; Bones, Jonathan. / Serum N-glycosylation in Parkinson’s disease : A novel approach for potential alterations. In: Molecules. 2019 ; Vol. 24, No. 12.
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