Serum levels and catabolism of 3'-azido-3'-deoxythymidine in vivo measured using a specific radioimmunoassay

Susan Cox, Ulla Rudén, K. Nagy, Jan Albert, Viveca Holmberg, Erik Sandström, Britta Wahren

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The thymidine analogue 3'-azido-3'-deoxythymidine is an effective inhibitor of HIV replication in vitro and is used in the treatment of acquired immunodeficiency syndrome. We report here upon a rapid sensitive radioimmunoassay for the detection of azidothymidine in serum or plasma. The assay is simple to perform and levels as low as 1 ng azidothymidine/ml can be detected. The assay is specific for azidothymidine and shows almost no cross-reaction with closely related nucleoside analogues or with naturally occurring nucleosides. Using this radioimmunoassay we were able to measure the azidothymidine levels in the serum of monkeys and acquired immunodeficiency syndrome patients treated with AZT. Individual variation in the peak serum level and clearance rate of azidothymidine were seen, which emphasizes the need to tailor the dose to the individual.

Original languageEnglish
Pages (from-to)89-98
Number of pages10
JournalJournal of Virological Methods
Volume30
Issue number1
DOIs
Publication statusPublished - 1990

Fingerprint

Zidovudine
Radioimmunoassay
Serum
Nucleosides
Acquired Immunodeficiency Syndrome
Cross Reactions
Thymidine
Haplorhini
HIV

Keywords

  • AIDS
  • Azidothymidine
  • Catabolism
  • Radioimmunoassay

ASJC Scopus subject areas

  • Virology

Cite this

Serum levels and catabolism of 3'-azido-3'-deoxythymidine in vivo measured using a specific radioimmunoassay. / Cox, Susan; Rudén, Ulla; Nagy, K.; Albert, Jan; Holmberg, Viveca; Sandström, Erik; Wahren, Britta.

In: Journal of Virological Methods, Vol. 30, No. 1, 1990, p. 89-98.

Research output: Contribution to journalArticle

Cox, Susan ; Rudén, Ulla ; Nagy, K. ; Albert, Jan ; Holmberg, Viveca ; Sandström, Erik ; Wahren, Britta. / Serum levels and catabolism of 3'-azido-3'-deoxythymidine in vivo measured using a specific radioimmunoassay. In: Journal of Virological Methods. 1990 ; Vol. 30, No. 1. pp. 89-98.
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