Serum amyloid P component induces TUNEL-positive nuclei in rat brain after intrahippocampal administration

Zoltán Urbányi, M. Sass, Judit Laszy, Viktor Takács, István Gyertyán, Tamás Pázmány

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Serum amyloid P component (SAP)-induced neuronal apoptosis has been demonstrated on the primary culture of embryonic rat cerebral cortex in vitro. Here we present pieces of evidence that cell death is also induced by serum amyloid P component in living rat brain similarly to that in cell culture. Intrahippocampally administered SAP diffuses from the site of injection to the cortical and subcortical area of the rat brain and enters the cells of brain tissue in 1 week. It induces elevation of the number of in situ TdT-mediated dUTP-X nick end-labeled nuclei in the hippocampus, cortex and subcortical structures of rat central nervous system. DNA fragmentation, which is detected by the end labeling reaction, is characteristic to apoptosis. It develops in 4 weeks following exposure. Apoptosis is an important form of cell death in different neurodegenerative diseases including Alzheimer's disease. Our present work reveals that apoptosis can be induced by SAP beyond other hitherto known apoptosis inducing components of neurodegeneration. Hereby SAP seems to be an important component of the process, which leads to expanded neuronal loss in the pathomechanism of neurodegenerative diseases.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalBrain Research
Volume1145
Issue number1
DOIs
Publication statusPublished - May 11 2007

Fingerprint

Serum Amyloid P-Component
In Situ Nick-End Labeling
Apoptosis
Brain
Neurodegenerative Diseases
Cell Death
DNA Fragmentation
Cerebral Cortex
Hippocampus
Alzheimer Disease
Central Nervous System
Cell Culture Techniques
Injections

Keywords

  • Apoptosis
  • DNA fragmentation
  • In vivo
  • Rat brain
  • Serum amyloid P component
  • TUNEL reaction

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Serum amyloid P component induces TUNEL-positive nuclei in rat brain after intrahippocampal administration. / Urbányi, Zoltán; Sass, M.; Laszy, Judit; Takács, Viktor; Gyertyán, István; Pázmány, Tamás.

In: Brain Research, Vol. 1145, No. 1, 11.05.2007, p. 221-226.

Research output: Contribution to journalArticle

Urbányi, Zoltán ; Sass, M. ; Laszy, Judit ; Takács, Viktor ; Gyertyán, István ; Pázmány, Tamás. / Serum amyloid P component induces TUNEL-positive nuclei in rat brain after intrahippocampal administration. In: Brain Research. 2007 ; Vol. 1145, No. 1. pp. 221-226.
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