Serum amyloid A in geese; cloning and expression of recombinant protein

Beáta Marianna Kovács, László Szilágyi, Janbaz Janan, P. Rudas

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We defined the nucleotide-sequence of the full-length goose serum amyloid A and compared it to SAA sequences of the duck. The aim of this work was to clone and express recombinant goose SAA and to produce antibody against this protein: Total RNA was isolated from goose liver and used to synthesise first strand cDNA. The coding region of the goose SAA cDNA was amplified by PCR using primers corresponding to the appropriate conservative regions of duck SAA mRNA. The product was subcloned into pET-15b expression vector to result in a His•Tag® fusion protein expression. The protein was purified by affinity chromatography. Rabbits were then immunized against the recombinant purified goose SAA protein. The anti-SAA serum was tested by Western blotting. Full-length goose SAA mRNA sequence has been obtained and sequenced.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalAmyloid
Volume12
Issue number2
DOIs
Publication statusPublished - Jun 2005

Fingerprint

Geese
Serum Amyloid A Protein
Recombinant Proteins
Organism Cloning
Ducks
Proteins
Complementary DNA
Messenger RNA
Affinity Chromatography
Clone Cells
Western Blotting
RNA
Rabbits
Polymerase Chain Reaction
Antibodies
Liver
Serum

Keywords

  • Cloning of the SAA gene
  • Geese
  • SAA
  • Serum amyloid A

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Serum amyloid A in geese; cloning and expression of recombinant protein. / Kovács, Beáta Marianna; Szilágyi, László; Janan, Janbaz; Rudas, P.

In: Amyloid, Vol. 12, No. 2, 06.2005, p. 109-114.

Research output: Contribution to journalArticle

Kovács, Beáta Marianna ; Szilágyi, László ; Janan, Janbaz ; Rudas, P. / Serum amyloid A in geese; cloning and expression of recombinant protein. In: Amyloid. 2005 ; Vol. 12, No. 2. pp. 109-114.
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