Serum albumin coating of demineralized bone matrix results in stronger new bone formation

Dénes B. Horváthy, Gabriella Vácz, Tamás Szabõ, Imola C. Szigyártõ, Ildikõ Torõ, Boglárka Vámos, István Hornyák, Károly Renner, Tamás Klára, Bence T. Szabõ, Csaba Dobõ-Nagy, Attila Doros, Zsombor Lacza

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15 Citations (Scopus)


Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we observed that significantly more cells adhere to the serum albumin coated DBMS at every time point. In vivo bone formation with albumin coated and uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By the seventh week, the bone defect in the albumin group was almost completely closed (remaining defect 3.0 ± 2.3%), while uncoated DBM and unfilled control groups still had significant defects (uncoated: 40.2 ± 9.1%, control: 52.4 ± 8.9%). Higher density values were also observed in the albumin coated DBM group. In addition, the serum albumin enhanced group showed significantly higher volume of newly formed bone in the microCT analysis and produced significantly higher breaking force and stiffness compared to the uncoated grafts (peak breaking force: uncoated: 15.7 ± 4 N, albumin 46.1 ± 11 N). In conclusion, this investigation shows that implanting serum albumin coated DBM significantly reduces healing period in nonhealing defects and results in mechanically stronger bone. These results also support the idea that serum albumin coating provides a convenient milieu for stem cell function, and a much improved bone grafting success can be achieved without the use of exogenous stem cells.

Original languageEnglish
Pages (from-to)126-132
Number of pages7
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number1
Publication statusPublished - Jan 1 2016


  • DBM
  • bone
  • calvaria defect
  • serum albumin
  • stem cells

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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    Horváthy, D. B., Vácz, G., Szabõ, T., Szigyártõ, I. C., Torõ, I., Vámos, B., Hornyák, I., Renner, K., Klára, T., Szabõ, B. T., Dobõ-Nagy, C., Doros, A., & Lacza, Z. (2016). Serum albumin coating of demineralized bone matrix results in stronger new bone formation. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 104(1), 126-132.