Serpins and the complement system

László Beinrohr, Thomas A. Murray-Rust, Leanne Dyksterhuis, Péter Závodszky, Péter Gál, Robert N. Pike, Lakshmi C. Wijeyewickrema

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

C1-inhibitor (serpin G1) is a 105 kDa inhibitor which functions as a major antiinflammatory protein in the body. It has its effects via inhibition of the proteases of the complement system and contact system of coagulation, as well as several direct effects mediated by its unique highly glycosylated N-terminal domain. The serpin controls a number of different proteases very efficiently and for some of these the function is augmented by the cofactor, heparin. Here, we describe the preparation of human plasma and recombinant C1-inhibitor and the basic methods required for their characterization, using the complement enzyme C1s as an example of a target enzyme.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages55-75
Number of pages21
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Enzymology
Volume499
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • C1-inhibitor
  • Complement proteases
  • Fermentation
  • Human plasma
  • Jacalin-agarose chromatography
  • Pichia pastoris
  • Recombinant protein expression
  • Serpin G1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Beinrohr, L., Murray-Rust, T. A., Dyksterhuis, L., Závodszky, P., Gál, P., Pike, R. N., & Wijeyewickrema, L. C. (2011). Serpins and the complement system. In Methods in Enzymology (pp. 55-75). (Methods in Enzymology; Vol. 499). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-386471-0.00004-3