SerpinB2 is involved in cellular response upon UV irradiation

Hajnalka Majoros, Zsuzsanna Ujfaludi, Barbara Nikolett Borsos, Viktória Vivien Hudacsek, Zita Nagy, Frederic Coin, Krisztina Buzas, I. Kovács, T. Bíró, I. Boros, Tibor Pankotai

Research output: Contribution to journalArticle

Abstract

Ultraviolet light induced pyrimidine dimer is a helix distortion DNA damage type, which recruits repair complexes. However, proteins of these complexes that take part in both DNA damage recognition and repair have been well-described, the regulation of the downstream steps of nucleotide excision repair (NER) have not been clearly clarified yet. In a high-throughput screen, we identified SerpinB2 (SPB2) as one of the most dramatically upregulated gene in keratinocytes following UV irradiation. We found that both the mRNA and the protein levels of SPB2 were increased upon UV irradiation in various cell lines. Additionally, UV damage induced translocation of SPB2 from the cytoplasm to the nucleus as well as the damage induced foci formation of it. Here we show that SPB2 co-localizes with XPB involved in the NER pathway at UV-induced repair foci. Finally, we demonstrated that UV irradiation promoted the association of SPB2 with ubiquitylated proteins. In basal cell carcinoma tumour cells, we identified changes in the subcellular localization of SPB2. Based on our results, we conclude that SPB2 protein has a novel role in UV-induced NER pathway, since it regulates the removal of the repair complex from the damaged site leading to cancerous malformation.

Original languageEnglish
Article number2753
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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DNA Repair
DNA Damage
Proteins
Pyrimidine Dimers
Basal Cell Carcinoma
Ultraviolet Rays
Keratinocytes
Cytoplasm
Cell Line
Messenger RNA
Genes
Neoplasms

ASJC Scopus subject areas

  • General

Cite this

Majoros, H., Ujfaludi, Z., Borsos, B. N., Hudacsek, V. V., Nagy, Z., Coin, F., ... Pankotai, T. (2019). SerpinB2 is involved in cellular response upon UV irradiation. Scientific reports, 9(1), [2753]. https://doi.org/10.1038/s41598-019-39073-w

SerpinB2 is involved in cellular response upon UV irradiation. / Majoros, Hajnalka; Ujfaludi, Zsuzsanna; Borsos, Barbara Nikolett; Hudacsek, Viktória Vivien; Nagy, Zita; Coin, Frederic; Buzas, Krisztina; Kovács, I.; Bíró, T.; Boros, I.; Pankotai, Tibor.

In: Scientific reports, Vol. 9, No. 1, 2753, 01.12.2019.

Research output: Contribution to journalArticle

Majoros, H, Ujfaludi, Z, Borsos, BN, Hudacsek, VV, Nagy, Z, Coin, F, Buzas, K, Kovács, I, Bíró, T, Boros, I & Pankotai, T 2019, 'SerpinB2 is involved in cellular response upon UV irradiation', Scientific reports, vol. 9, no. 1, 2753. https://doi.org/10.1038/s41598-019-39073-w
Majoros H, Ujfaludi Z, Borsos BN, Hudacsek VV, Nagy Z, Coin F et al. SerpinB2 is involved in cellular response upon UV irradiation. Scientific reports. 2019 Dec 1;9(1). 2753. https://doi.org/10.1038/s41598-019-39073-w
Majoros, Hajnalka ; Ujfaludi, Zsuzsanna ; Borsos, Barbara Nikolett ; Hudacsek, Viktória Vivien ; Nagy, Zita ; Coin, Frederic ; Buzas, Krisztina ; Kovács, I. ; Bíró, T. ; Boros, I. ; Pankotai, Tibor. / SerpinB2 is involved in cellular response upon UV irradiation. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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