Serotonin may alter the pattern of gonadotropin-induced progesterone release of human granulosa cells in superfusion system

M. Koppán, Jozsef Bodis, Zsofia Verzar, Hans Rudolf Tinneberg, Attila Torok

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Serotonin plays a hormonal function in several nonneuronal peripheral tissues, such as the ovaries. Our aim was to investigate whether there is a modulatory action of serotonin on gonadotropin-induced steroid secretion of human granulosa cells. In granulosa cell culture, serotonin was administered alone or in combination with luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Also, granulosa cells were transferred into a dynamic superfusion apparatus and challenged by FSH and LH alone or along with serotonin. Estradiol and progesterone concentrations of samples were measured by radioimmunoassay. As expected, administration of FSH, LH, and serotonin alone resulted in a significant estradiol and progesterone release in cell culture, as well as a significant increase in progesterone release in dynamic superfusion system. In cell culture, co-administration of serotonin with gonadotropins had no additive effect on gonadotropin-induced secretion of progesterone, while it further augmented that of estradiol. In superfusion system, when gonadotropins were added along with serotonin, the increase in progesterone release was markedly less, while peaks of hormone response were remarkably prolonged compared to challenges by LH and FSH alone. The observed effects of serotonin on gonadotropin-induced steroid release of granulosa cells may reveal further details about the regulation of granulosa cell function.

Original languageEnglish
Pages (from-to)155-159
Number of pages5
JournalEndocrine
Volume24
Issue number2
DOIs
Publication statusPublished - Jul 2004

Fingerprint

Granulosa Cells
Gonadotropins
Progesterone
Serotonin
Follicle Stimulating Hormone
Luteinizing Hormone
Estradiol
Cell Culture Techniques
Steroids
Radioimmunoassay
Ovary
Hormones

Keywords

  • Cell culture
  • Estradiol
  • FSH
  • Granulosa cell superfusion
  • LH
  • Progesterone
  • Serotonin

ASJC Scopus subject areas

  • Endocrinology

Cite this

Serotonin may alter the pattern of gonadotropin-induced progesterone release of human granulosa cells in superfusion system. / Koppán, M.; Bodis, Jozsef; Verzar, Zsofia; Tinneberg, Hans Rudolf; Torok, Attila.

In: Endocrine, Vol. 24, No. 2, 07.2004, p. 155-159.

Research output: Contribution to journalArticle

Koppán, M. ; Bodis, Jozsef ; Verzar, Zsofia ; Tinneberg, Hans Rudolf ; Torok, Attila. / Serotonin may alter the pattern of gonadotropin-induced progesterone release of human granulosa cells in superfusion system. In: Endocrine. 2004 ; Vol. 24, No. 2. pp. 155-159.
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