Serotonin-immunoreactive and dopamine-immunoreactive neurones in the terminal ganglion of the cricket, Acheta domestica: Light- and electron-microscopic immunocytochemistry

Károly Elekes, Reinhold Hustert, Michel Geffard

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The distribution and ultrastructure of serotonin- and dopamine-immunoreactive (5-HTi and DAi) neurones have been investigated in the terminal ganglion of the cricket, Acheta domestica, using a pre-embedding chopper technique. Special attention has been paid to the immunoreactive structures in the neuropil. 5-HTi structures are extensively distributed and densely packed throughout the 5 neuromeres of the terminal ganglion and originate from several interneurones and efferent neurones. In contrast, DAi fibres are distributed sparsely although they extend to all neuromeres of the ganglion and originate from 6 interneurons only. For both 5-HTi and DAi neurones characteristic axonal projections and branching patterns can be distinguished. The 5-HTi axons exhibit rich varicose arborizations, whereas DAi neurones possess fewer varicosities in the neuropil. Electron microscopy shows that 5-HTi varicosities contain small (∼ 60 nm) and large (∼ 100 nm) agranular vesicles, and large (∼ 100 nm) granular vesicles, whereas in DAi varicosities small (∼ 60 nm) agranular and large (∼ 100 nm) granular vesicles are seen. Both 5-HTi and DAi varicosities form synaptic contacts. We conclude that both serotonin and dopamine may be used as neurotransmitters in the terminal ganglion of the cricket.

Original languageEnglish
Pages (from-to)167-180
Number of pages14
JournalCell And Tissue Research
Volume250
Issue number1
DOIs
Publication statusPublished - Oct 1 1987

Keywords

  • Acheta domestica
  • Dopamin
  • Immunocytochemistry
  • Serotonin
  • Terminal ganglion

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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