Serotonin content is elevated in the immune cells of histidine decarboxylase gene knock-out (HDCKO) mice. Focus on mast cells

G. Csaba, P. Kovács, E. Búzás, M. Mazán, E. Pállinger

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: Biogenic amines, histamine and serotonin are present in the granules and nucleus of mast cells. We wanted to study the presence, amount and localization of serotonin in mast cells and other cells of the immune system, under conditions of histamine deficiency caused by knock out of histamine decarboxylase gene (HDCKO). Methods: Wild type and histamine deficient HDCKO mice were studied for serotonin content of the immune cells (lymphocytes as well as the monocyte-granulocyte-mast cell group) using flow cytometry and confocal microscopy. Groups of mice were kept either on complete rodent chow or on a histamine-free diet for a month before the experiments. Results: The amount of serotonin was significantly higher in the KO animals, irrespective of the diet. Confocal microscopy demonstrated the presence of serotonin in the nucleus of mast cells in the wild type animals, while it was not present in the KO mice. Furthermore, in the cytoplasm (granules) of KO mast cells a bright fluorescence was observed in contrast to the pale fluorescence of wild animals. Conclusion: It seems likely that serotonin replaces the deficient histamine in the heparin-biogenic amine complex in the mast cell granules.

Original languageEnglish
Pages (from-to)89-92
Number of pages4
JournalInflammation Research
Volume56
Issue number2
DOIs
Publication statusPublished - Feb 2007

Fingerprint

Histidine Decarboxylase
Gene Knockout Techniques
Knockout Mice
Mast Cells
Histamine
Serotonin
Genes
Animals
Biogenic Amines
Wild Animals
Confocal microscopy
Nutrition
Confocal Microscopy
Fluorescence
Diet
Carboxy-Lyases
Lymphocytes
Flow cytometry
Immune system
Granulocytes

Keywords

  • Gene knock-out
  • Histamine
  • Mast cell
  • Serotonin
  • White blood cells

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)
  • Immunology
  • Cell Biology

Cite this

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title = "Serotonin content is elevated in the immune cells of histidine decarboxylase gene knock-out (HDCKO) mice. Focus on mast cells",
abstract = "Objective: Biogenic amines, histamine and serotonin are present in the granules and nucleus of mast cells. We wanted to study the presence, amount and localization of serotonin in mast cells and other cells of the immune system, under conditions of histamine deficiency caused by knock out of histamine decarboxylase gene (HDCKO). Methods: Wild type and histamine deficient HDCKO mice were studied for serotonin content of the immune cells (lymphocytes as well as the monocyte-granulocyte-mast cell group) using flow cytometry and confocal microscopy. Groups of mice were kept either on complete rodent chow or on a histamine-free diet for a month before the experiments. Results: The amount of serotonin was significantly higher in the KO animals, irrespective of the diet. Confocal microscopy demonstrated the presence of serotonin in the nucleus of mast cells in the wild type animals, while it was not present in the KO mice. Furthermore, in the cytoplasm (granules) of KO mast cells a bright fluorescence was observed in contrast to the pale fluorescence of wild animals. Conclusion: It seems likely that serotonin replaces the deficient histamine in the heparin-biogenic amine complex in the mast cell granules.",
keywords = "Gene knock-out, Histamine, Mast cell, Serotonin, White blood cells",
author = "G. Csaba and P. Kov{\'a}cs and E. B{\'u}z{\'a}s and M. Maz{\'a}n and E. P{\'a}llinger",
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language = "English",
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T1 - Serotonin content is elevated in the immune cells of histidine decarboxylase gene knock-out (HDCKO) mice. Focus on mast cells

AU - Csaba, G.

AU - Kovács, P.

AU - Búzás, E.

AU - Mazán, M.

AU - Pállinger, E.

PY - 2007/2

Y1 - 2007/2

N2 - Objective: Biogenic amines, histamine and serotonin are present in the granules and nucleus of mast cells. We wanted to study the presence, amount and localization of serotonin in mast cells and other cells of the immune system, under conditions of histamine deficiency caused by knock out of histamine decarboxylase gene (HDCKO). Methods: Wild type and histamine deficient HDCKO mice were studied for serotonin content of the immune cells (lymphocytes as well as the monocyte-granulocyte-mast cell group) using flow cytometry and confocal microscopy. Groups of mice were kept either on complete rodent chow or on a histamine-free diet for a month before the experiments. Results: The amount of serotonin was significantly higher in the KO animals, irrespective of the diet. Confocal microscopy demonstrated the presence of serotonin in the nucleus of mast cells in the wild type animals, while it was not present in the KO mice. Furthermore, in the cytoplasm (granules) of KO mast cells a bright fluorescence was observed in contrast to the pale fluorescence of wild animals. Conclusion: It seems likely that serotonin replaces the deficient histamine in the heparin-biogenic amine complex in the mast cell granules.

AB - Objective: Biogenic amines, histamine and serotonin are present in the granules and nucleus of mast cells. We wanted to study the presence, amount and localization of serotonin in mast cells and other cells of the immune system, under conditions of histamine deficiency caused by knock out of histamine decarboxylase gene (HDCKO). Methods: Wild type and histamine deficient HDCKO mice were studied for serotonin content of the immune cells (lymphocytes as well as the monocyte-granulocyte-mast cell group) using flow cytometry and confocal microscopy. Groups of mice were kept either on complete rodent chow or on a histamine-free diet for a month before the experiments. Results: The amount of serotonin was significantly higher in the KO animals, irrespective of the diet. Confocal microscopy demonstrated the presence of serotonin in the nucleus of mast cells in the wild type animals, while it was not present in the KO mice. Furthermore, in the cytoplasm (granules) of KO mast cells a bright fluorescence was observed in contrast to the pale fluorescence of wild animals. Conclusion: It seems likely that serotonin replaces the deficient histamine in the heparin-biogenic amine complex in the mast cell granules.

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