Sequential injection setup for capillary isoelectric focusing combined with MS detection

Csilla Páger, Ágnes Dörnyei, F. Kilár

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Capillary isoelectric focusing in the presence of electroosmosis with sequential injection of carrier ampholytes and sample was found to be suitable for MS detection. The separate injection of the sample and the ampholytes provides good condition to suppress and overcome the undesirable effect of the presence of ampholytes in MS. By the appropriate selection of ampholyte solutions, whose pH range not necessarily covers the pI values of the analytes, the migration of the components can be controlled, and the impact of the ampholytes on MS detection is decreased. The unique applicability of this setup is shown by testing several parameters, such as the application of volatile electrolyte solutions, the type of the ampholytes, the order and the number of the ampholyte and sample zones. Broad and narrow pH range ampholytes were applied in experiments using uncoated capillaries with different lengths for the analyses of substituted nitrophenol dyes to achieve optimal conditions for the MS detection. Although the sample components are not leaving the pH gradient, due to the decrease in the ampholyte concentration at the position of the components, and because the sample components migrate in charged state, the ionisation is more effective for MS detection.

Original languageEnglish
Pages (from-to)1875-1884
Number of pages10
JournalElectrophoresis
Volume32
Issue number14
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Isoelectric Focusing
Buffers
Injections
Ampholyte Mixtures
Electroosmosis
Nitrophenols
Proton-Motive Force
Electrolytes
Ionization
Coloring Agents
Testing

Keywords

  • Capillary isoelectric focusing
  • Carrier ampholytes
  • MS detection
  • PI markers
  • Sequential injection

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Sequential injection setup for capillary isoelectric focusing combined with MS detection. / Páger, Csilla; Dörnyei, Ágnes; Kilár, F.

In: Electrophoresis, Vol. 32, No. 14, 07.2011, p. 1875-1884.

Research output: Contribution to journalArticle

Páger, Csilla ; Dörnyei, Ágnes ; Kilár, F. / Sequential injection setup for capillary isoelectric focusing combined with MS detection. In: Electrophoresis. 2011 ; Vol. 32, No. 14. pp. 1875-1884.
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