Sequential centrifugal layer chromatography (SCLC), a new preparative planar separation technique, combines the advantages of preparative centrifugal layer chromatography (CLC) and analytical, horizontal, anticircular, and sequential thin‐layer chromatography (TLC). In this on‐line preparative method, solvent delivery is locally and temporally variable, which means that the mobile phase can be brought onto the plate at any desired place. The SCLC apparatus operates in two modes: circular, with the help of centrifugal force in an inclined position, and anticircular, by means of capillary action in a horizontal position. These two operation modes can be combined and repeated as often as required, hence the separation space becomes practically unlimited. In this way the capacity and separation performance is significantly increased. In the present paper the apparatus and the method are described and some practical aspects are mentioned.
- Anticircular development
- Centrifugal layer chromatography, CLC
- Circular development
- Preparative planar chromatography, PPC
- Sequential centrifugal layer chromatography, SCLC
ASJC Scopus subject areas
- Chemical Engineering(all)