Sequential activation of p75 and TrkB is involved in dendritic development of subventricular zone-derived neuronal progenitors in vitro

E. Gascon, L. Vutskits, H. Zhang, M. J. Barral-Moran, P. J. Kiss, C. Mas, J. Kiss

Research output: Contribution to journalArticle

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Abstract

Dendritic arbor development of subventricular zone-derived interneurons is a critical step in their integration into functional circuits of the postnatal olfactory bulb. However, the mechanism and molecular control of this process remain unknown. In this study, we have developed a culture model where dendritic development of purified subventricular zone cells proceeds under serum-free conditions in the absence of added growth factors and non-neural cells. We demonstrate that the large majority of these cells in culture express GABA and elaborate dendritic arbors with spine-like protrusions but they do not possess axons. These neurons expressed receptors for neurotrophins including p75, TrkB and TrkC but not TrkA. Application of exogenous neurotrophins, including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3) and nerve growth factor (NGF), to cultures stimulated dendritic growth and led to more complex dendritic arbors during the initial 3 days in culture. Our results suggest that these effects are independent of Trk receptors and mediated by the p75/ceramide signaling pathway. We also show that brain-derived neurotrophic factor is the only neurotrophin that is able to influence late-phase dendritic development via TrkB receptor activation. These results suggest that dendritic arbor development of subventricular zone-derived cells may be regulated by neurotrophins through the activation of p75 and the TrkB receptor signaling pathways in a sequentially defined temporal pattern.

Original languageEnglish
Pages (from-to)69-80
Number of pages12
JournalEuropean Journal of Neuroscience
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Lateral Ventricles
Nerve Growth Factors
trkB Receptor
Brain-Derived Neurotrophic Factor
Neurotrophin 3
Nerve Growth Factor Receptor
Ceramides
Olfactory Bulb
Nerve Growth Factor
Interneurons
gamma-Aminobutyric Acid
Axons
Intercellular Signaling Peptides and Proteins
Spine
Cell Culture Techniques
Neurons
Growth
Serum
In Vitro Techniques

Keywords

  • Dendrite
  • Olfactory interneurons
  • Rat
  • Subventricular zone
  • Trk receptors

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sequential activation of p75 and TrkB is involved in dendritic development of subventricular zone-derived neuronal progenitors in vitro. / Gascon, E.; Vutskits, L.; Zhang, H.; Barral-Moran, M. J.; Kiss, P. J.; Mas, C.; Kiss, J.

In: European Journal of Neuroscience, Vol. 21, No. 1, 01.2005, p. 69-80.

Research output: Contribution to journalArticle

Gascon, E. ; Vutskits, L. ; Zhang, H. ; Barral-Moran, M. J. ; Kiss, P. J. ; Mas, C. ; Kiss, J. / Sequential activation of p75 and TrkB is involved in dendritic development of subventricular zone-derived neuronal progenitors in vitro. In: European Journal of Neuroscience. 2005 ; Vol. 21, No. 1. pp. 69-80.
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