Sequencing-independent method for the differentiation of the main phylogenetic lineages of Pasteurella multocida

Boglárka Sellyei, E. Wehmann, Tibor Magyar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A rapid, easy method involving a polymerase chain reaction (PCR) assay followed by restriction fragment length polymorphism (RFLP) analysis was developed to differentiate the 2 phylogenetic lineages of Pasteurella multocida. The PCR targeted the 16S ribosomal RNA gene, and the RFLP involved separate digestions with HindIII, EarI, and MlsI. The method was applied to 16 isolates of P. multocida from different hosts and the isolates were clearly assigned to 1 of the 2 lineages. The phylogenetic lineages did not match with the phenotypic-based identification at the subspecies level.

Original languageEnglish
Pages (from-to)735-738
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume24
Issue number4
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Pasteurella multocida
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
polymerase chain reaction
16S Ribosomal RNA
Polymerase Chain Reaction
phylogeny
rRNA Genes
rapid methods
Digestion
digestion
ribosomal RNA
assays
methodology
genes

Keywords

  • Lineage
  • Pasteurella multocida
  • polymerase chain reaction
  • restriction fragment length polymorphism

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Sequencing-independent method for the differentiation of the main phylogenetic lineages of Pasteurella multocida. / Sellyei, Boglárka; Wehmann, E.; Magyar, Tibor.

In: Journal of Veterinary Diagnostic Investigation, Vol. 24, No. 4, 07.2012, p. 735-738.

Research output: Contribution to journalArticle

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