Sequence variation in the melanocortin-1 receptor gene (Mc1r) does not explain variation in the degree of melanism in a widespread amphibian

G. Herczeg, Chikako Matsuba, Juha Merilä

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Variation in nucleotide sequence of the melanocortin-1 receptor gene (Mc1r) is associated with melanism in several mammalian, avian and reptilian species, but no attempts have been made to understand the genetic underpinnings of melanism in amphibians. We isolated the complete coding sequence (945 bps) of Mc1r from the common frog (Rana temporaria) and compared the predicted amino acid sequence with that of fish, reptiles, birds and mammals. We investigated associations between nucleotide substitutions and the level of dorsal melanism among 28 individuals from two populations with pronounced differences in melanism. According to our results, The transmembrane regions of Mc1r are conserved across vertebrates. In the population comparison, we only found five nucleotide sites with synonymous substitutions; none is being associated with the level of melanism. Our results suggest that either other genes or regulatory regions outside the coding sequence of Mc1r are responsible for expression of melanism in R. temporaria.

Original languageEnglish
Pages (from-to)37-45
Number of pages9
JournalAnnales Zoologici Fennici
Volume47
Issue number1
Publication statusPublished - Mar 2010

Fingerprint

melanism
alpha-melanocyte-stimulating hormone
amphibian
amphibians
receptors
gene
Rana temporaria
genes
nucleotides
substitution
reptiles
frogs
reptile
frog
amino acid sequences
vertebrates
sequence diversity
mammals
vertebrate
mammal

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Nature and Landscape Conservation

Cite this

Sequence variation in the melanocortin-1 receptor gene (Mc1r) does not explain variation in the degree of melanism in a widespread amphibian. / Herczeg, G.; Matsuba, Chikako; Merilä, Juha.

In: Annales Zoologici Fennici, Vol. 47, No. 1, 03.2010, p. 37-45.

Research output: Contribution to journalArticle

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