Sequence and homology model of 3-isopropylmalate dehydrogenase from the psychrotrophic bacterium Vibrio sp. I5 suggest reasons for thermal instability

Gerlind Wallon, Susan T. Lovett, Csaba Magyar, Adam Svingor, Andras Szilagyi, Pèter Zàvodszky, Dagmar Ringe, Gregory A. Petsko

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The leuB gene from the psychrotrophic strain Vibrio sp. I5 has been cloned and sequenced. The gene codes for 3-isopropylmalate dehydrogenase, a 360-residue, dimeric enzyme involved in the biosynthesis of leucine. Three recently solved homologous isopropylmalate dehydrogenase (IPMDH) crystal structures from thermophilic and mesophilic organisms have been used to build a homology model for the psychrotrophic IPMDH and to deduce the possible structural reasons for its decreased thermostability. According to our model the psychrotrophic IPMDH contains fewer stabilizing interactions than its mesophilic and thermophilic counterparts. Elements that have been identified as destabilizing in the comparison of the psychrotrophic, mesophilic and thermophilic IPMDHs are a smaller number of salt-bridges, a reduction in aromatic-aromatic interactions, fewer proline residues and longer surface loops. In addition, there are a number of substitutions of otherwise strictly conserved residues that can be linked to thermostability.

Original languageEnglish
Pages (from-to)665-672
Number of pages8
JournalProtein Engineering
Volume10
Issue number6
Publication statusPublished - Jun 1 1997

    Fingerprint

Keywords

  • Cold adaptation
  • Homology model
  • Isopropylmalate dehydrogenase
  • Psychrotroph
  • Sequence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this