Sequence analysis of the VP7 and VP4 genes identifies a novel VP7 gene allele of porcine rotaviruses, sharing a common evolutionary origin with human G2 rotaviruses

V. Martella, M. Ciarlet, R. Baselga, S. Arista, G. Elia, E. Lorusso, K. Bányai, V. Terio, A. Madio, F. M. Ruggeri, E. Falcone, M. Camero, N. Decaro, C. Buonavoglia

Research output: Contribution to journalArticle

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Abstract

During an epidemiological survey encompassing several porcine herds in Saragoza, Spain, the VP7 and VP4 of a rotavirus-positive sample, 34461-4, could not be predicted by using multiple sets of G- and P-type-specific primers. Sequence analysis of the VP7 gene revealed a low amino acid (aa) identity with those of well-established G serotypes, ranging between 58.33% and 88.88%, with the highest identity being to human G2 rotaviruses. Analysis of the VP4 gene revealed a P[23] VP4 specificity, as its VP8* aa sequence was 95.9% identical to that of the P14[23],G5 porcine strain A34, while analysis of the VP6 indicated a genogroup I, that is predictive of subgroup I specificity. Analysis of the 10th and 11th RNA segments revealed close identity to strains of porcine and human origin, respectively. The relatively low overall aa sequence conservation (

Original languageEnglish
Pages (from-to)111-123
Number of pages13
JournalVirology
Volume337
Issue number1
DOIs
Publication statusPublished - Jun 20 2005

Fingerprint

Rotavirus
Sequence Analysis
Swine
Alleles
Amino Acid Sequence
Pyridinolcarbamate
Genes
Spain
Genotype
RNA
Amino Acids

Keywords

  • G-type
  • P-type
  • Rotavirus
  • VP4
  • VP7

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Sequence analysis of the VP7 and VP4 genes identifies a novel VP7 gene allele of porcine rotaviruses, sharing a common evolutionary origin with human G2 rotaviruses. / Martella, V.; Ciarlet, M.; Baselga, R.; Arista, S.; Elia, G.; Lorusso, E.; Bányai, K.; Terio, V.; Madio, A.; Ruggeri, F. M.; Falcone, E.; Camero, M.; Decaro, N.; Buonavoglia, C.

In: Virology, Vol. 337, No. 1, 20.06.2005, p. 111-123.

Research output: Contribution to journalArticle

Martella, V, Ciarlet, M, Baselga, R, Arista, S, Elia, G, Lorusso, E, Bányai, K, Terio, V, Madio, A, Ruggeri, FM, Falcone, E, Camero, M, Decaro, N & Buonavoglia, C 2005, 'Sequence analysis of the VP7 and VP4 genes identifies a novel VP7 gene allele of porcine rotaviruses, sharing a common evolutionary origin with human G2 rotaviruses', Virology, vol. 337, no. 1, pp. 111-123. https://doi.org/10.1016/j.virol.2005.03.031
Martella, V. ; Ciarlet, M. ; Baselga, R. ; Arista, S. ; Elia, G. ; Lorusso, E. ; Bányai, K. ; Terio, V. ; Madio, A. ; Ruggeri, F. M. ; Falcone, E. ; Camero, M. ; Decaro, N. ; Buonavoglia, C. / Sequence analysis of the VP7 and VP4 genes identifies a novel VP7 gene allele of porcine rotaviruses, sharing a common evolutionary origin with human G2 rotaviruses. In: Virology. 2005 ; Vol. 337, No. 1. pp. 111-123.
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