Sequence analysis and deletion of porcine adenovirus serotype 5 E3 region

T. Tuboly, É Nagy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A 3000 basepair (bp) region corresponding to the E3 region, the flanking pVIII and part of the fiber protein genes, of the two prototype strains (HNF- 61 and HNF-70) of porcine adenovirus serotype five (PAdV-5) was sequenced. A potential E3 promoter and poly-A signals were identified. The size of the E3 region was 2039 (strain HNF-61) and 2020 bp (strain HNF-70) the largest E3 so far reported among PAdVs. Three open reading frames (ORF2-4) were identified within the E3 region. Based on the predicted amino acid (aa) sequences ORF2 was similar to other adenovirus E3 ORFs, ORF3 showed some similarity to a bovine adenovirus (BAdV-1) ORF. ORF4 was unique to PAdV-5. E3 mRNA transcripts were detected early in infection by Northern blot analysis. Genomic clones of HNF-70 with a 1505 or 1237 bp deletions in the E3 region were constructed to map non-essential regions. After transfection of the DNA into swine testicle cells, virions were recovered for only the shorter 1237 bp deletion. At least 60% of the E3 region was not essential for virus replication, bringing the theoretical vector capacity of a helper independent PAdV-5 to 2.9 kb. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)109-117
Number of pages9
JournalVirus Research
Volume68
Issue number2
DOIs
Publication statusPublished - Jul 10 2000

Fingerprint

Porcine Adenoviruses
Sequence Deletion
Open Reading Frames
Sequence Analysis
Adenoviridae
Poly A
Virus Replication
Northern Blotting
Virion
Transfection
Testis
Amino Acid Sequence
Swine
Clone Cells
Messenger RNA
DNA
Infection
Serogroup
Proteins

Keywords

  • Deletion mutant
  • E3 region
  • Porcine adenovirus serotype 5

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Sequence analysis and deletion of porcine adenovirus serotype 5 E3 region. / Tuboly, T.; Nagy, É.

In: Virus Research, Vol. 68, No. 2, 10.07.2000, p. 109-117.

Research output: Contribution to journalArticle

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