Seperation of 125I-labelled derivatives of 5-hydroxy-6,8,11,14-ecosatetraenoic acid

I. Mucha, Ildikó Paluska-Ferencz, G. Tóth

Research output: Contribution to journalArticle

1 Citation (Scopus)


Monoiodinated tyrosine methyl ester, a derivative of 5-hydroxy-6,8,11,14-eicosatetraenic acid containing 125I in the phenolic orhto position was prepared with high specific radioactivity and separated by column chromatography on a Sephadex LH-20 gel. The adsorption behaviour of the labelled product was studied by absorption chromatography suing a Sephadex LH-20 adsorbent with ethanol-water as a binary eluent and by reversed-phase high-performance liquid chromatograhy using C18-silica as a stationary phase with aqueous binary containing ethanol, methanol or acetonitrile. In both separation systems, a linear relationship was found between the logarithmic capacity factor or distribution coefficient and the logarithmic concentration of the organic solvent.

Original languageEnglish
Pages (from-to)307-314
Number of pages8
JournalJournal of Chromatography A
Issue numberC
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Seperation of <sup>125</sup>I-labelled derivatives of 5-hydroxy-6,8,11,14-ecosatetraenoic acid'. Together they form a unique fingerprint.

  • Cite this