Seperation of 125I-labelled derivatives of 5-hydroxy-6,8,11,14-ecosatetraenoic acid

I. Muchá, Ildikó Paluska-Ferencz, G. Tóth

Research output: Contribution to journalArticle

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Abstract

Monoiodinated tyrosine methyl ester, a derivative of 5-hydroxy-6,8,11,14-eicosatetraenic acid containing 125I in the phenolic orhto position was prepared with high specific radioactivity and separated by column chromatography on a Sephadex LH-20 gel. The adsorption behaviour of the labelled product was studied by absorption chromatography suing a Sephadex LH-20 adsorbent with ethanol-water as a binary eluent and by reversed-phase high-performance liquid chromatograhy using C18-silica as a stationary phase with aqueous binary containing ethanol, methanol or acetonitrile. In both separation systems, a linear relationship was found between the logarithmic capacity factor or distribution coefficient and the logarithmic concentration of the organic solvent.

Original languageEnglish
Pages (from-to)307-314
Number of pages8
JournalJournal of Chromatography A
Volume543
Issue numberC
DOIs
Publication statusPublished - 1991

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Chromatography
Ethanol
Derivatives
Column chromatography
Acids
Radioactivity
Silicon Dioxide
Organic solvents
Adsorbents
Adsorption
Methanol
Gels
Water
Liquids
sephadex
acetonitrile
tyrosine methyl ester

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Seperation of 125I-labelled derivatives of 5-hydroxy-6,8,11,14-ecosatetraenoic acid. / Muchá, I.; Paluska-Ferencz, Ildikó; Tóth, G.

In: Journal of Chromatography A, Vol. 543, No. C, 1991, p. 307-314.

Research output: Contribution to journalArticle

Muchá, I. ; Paluska-Ferencz, Ildikó ; Tóth, G. / Seperation of 125I-labelled derivatives of 5-hydroxy-6,8,11,14-ecosatetraenoic acid. In: Journal of Chromatography A. 1991 ; Vol. 543, No. C. pp. 307-314.
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