Seperation of 125I-labelled derivatives of 5-hydroxy-6,8,11,14-ecosatetraenoic acid

I. Mucha, Ildikó Paluska-Ferencz, G. Tóth

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1 Citation (Scopus)

Abstract

Monoiodinated tyrosine methyl ester, a derivative of 5-hydroxy-6,8,11,14-eicosatetraenic acid containing 125I in the phenolic orhto position was prepared with high specific radioactivity and separated by column chromatography on a Sephadex LH-20 gel. The adsorption behaviour of the labelled product was studied by absorption chromatography suing a Sephadex LH-20 adsorbent with ethanol-water as a binary eluent and by reversed-phase high-performance liquid chromatograhy using C18-silica as a stationary phase with aqueous binary containing ethanol, methanol or acetonitrile. In both separation systems, a linear relationship was found between the logarithmic capacity factor or distribution coefficient and the logarithmic concentration of the organic solvent.

Original languageEnglish
Pages (from-to)307-314
Number of pages8
JournalJournal of Chromatography A
Volume543
Issue numberC
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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