Human serum transferrin (Tf), a glycoprotein, has two lobes, each having a binding site for trivalent iron ions. The binding of metal ions and different anions (oxalate, aziridine-carboxylate, bicarbonate) to these binding site results four different molecular form of Tf (holo-Tf, diferri FeNTfFeC, and monoferri TfFeN, TfFeC). High performance capillary zone electrophoresis (CZE) experiments were performed in order to separate these molecular forms. We changed the following separation conditions: background electrolyte, capillary treatment and voltage to achieve high efficiency of separation of transferrin probes after the complexation reaction.
|Number of pages||12|
|Journal||UPB Scientific Bulletin, Series B: Chemistry and Materials Science|
|Publication status||Published - Aug 23 2012|
- Capillary zone electrophoresis
ASJC Scopus subject areas
- Materials Science(all)