Separation of urea and other polar compounds by HPLC

M. Pálfi-Ledniczky, G. Dénes, K. Ujszászy

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Abstract

Urea, glycolic and aminoxyacetic acid amides are the polar metabolites of 2-acetyl-3-phenyl-tetrahydro-1,2,4-oxadiazin-5-one (RGH 4615). They cannot be separated on octadecyl-, cyanosilyl- or amino-phase columns, but silica, used with C3-C4 alcohol and water mixtures as the eluent permits their separation. Besides refractive index detection and on-line radioactivity measurement the metabolic formation of14C-urea was proved by enzymatic cleavage into14CO2.

Original languageEnglish
Pages (from-to)847-848
Number of pages2
JournalChromatographia
Volume24
Issue number1
DOIs
Publication statusPublished - Dec 1 1987

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Keywords

  • Column liquid chromatography
  • Silica phase
  • Urea

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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