Separation of the human transferrin forms by carrier- free high-performance zone electrophoresis and isoelectric focusing

F. Kilár, Stellan Hjertén

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Human transferrin isoforms, i.e., molecules with different carbohydrate contents which differ each other by only one negative charge, were resolved by high-performance zone electrophoresis in free solution. The di-, tetra-, penta-, hexa- and heptasialo transferrins could be asssigned in the electrophoretic pattern. The pattern changed when iron-free transferrin was treated with neuraminidase, which splits off the sialic acid from the carbohydrate chains. The final digest contained transferrin molecules without sialic acids, as was confirmed by isoelectric focusing.

Original languageEnglish
Pages (from-to)351-357
Number of pages7
JournalJournal of Chromatography A
Volume480
Issue numberC
DOIs
Publication statusPublished - 1989

Fingerprint

Isoelectric Focusing
Transferrin
Electrophoresis
Transferrins
Carbohydrates
Sialic Acids
Rubiaceae
Molecules
Neuraminidase
N-Acetylneuraminic Acid
Protein Isoforms
Iron

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Separation of the human transferrin forms by carrier- free high-performance zone electrophoresis and isoelectric focusing. / Kilár, F.; Hjertén, Stellan.

In: Journal of Chromatography A, Vol. 480, No. C, 1989, p. 351-357.

Research output: Contribution to journalArticle

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