The objective of the work described in this paper was to investigate tThe separation of ginsenosides by TLC, HPTLC, and OPLC. The best separation of the ginsenosides was obtained by use of OPLC on silica gel 60F254 with chloroform-methanol-ethyl acetate-water-hexane, 20 + 22 + 60 + 8 + 4 (v/v), as mobile phase. Results from TLC, HPTLC, and OPLC analysis of ginsenosides indicated that use of the different methods of development resulted in significant differences between the resolution of the compounds. Forced-flow OPLC is the most sensitive method for analysis of the ginsenosides.
- High-performance thin-layer chromatograhy, HPTLC
- Overpressured-layer chromatography, OPLC
- Panax quinquefolium L.
- Thin-layer chromatography, TLC
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry