Separation of humic acids using capillary isoelectric focusing

Piroska Kovács, József Posta

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Humic acids are natural polymers occurring in soils and sediments. These molecules modify the structure of soil and sediment and bind metal ions but because of structural diversity analysis of humic acids is difficult. Capillary isoelectric focusing (CIEF) was first applied in this field by Schmitt et al. but they obtained 3-4 fractions only. The aim of present study was to develop a new and reproducible CIEF method for the separation of humic acids with better resolution. Commercial humic acid samples purchased from Fluka and Aldrich and a peat humic acid were used for method development and comparative studies. Methyl cellulose and polyvinyl alcohol (PVA) were used to increase resolution. Reduced Triton X-100, SDS and urea were applied as anticoagulants. Effects of additives on resolution and reproducibility were compared in different concentrations and optimum concentrations were found to be as follows: 0.2% methyl cellulose, 0.5% PVA and 0.3% reduced Triton X-100. Thus, 30-50 stable fractions could be obtained depending on the sample.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalMicrochemical Journal
Volume79
Issue number1-2
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Humic Substances
Polyvinyl Alcohol
Methylcellulose
Octoxynol
Sediments
Natural polymers
Soils
Peat
Anticoagulants
Metal ions
Urea
Isoelectric Focusing
Molecules

Keywords

  • Capillary electrophoresis
  • Capillary isoelectric focusing
  • Humic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Separation of humic acids using capillary isoelectric focusing. / Kovács, Piroska; Posta, József.

In: Microchemical Journal, Vol. 79, No. 1-2, 01.2005, p. 49-54.

Research output: Contribution to journalArticle

@article{ca17543e20f042179f0410c08251eaf2,
title = "Separation of humic acids using capillary isoelectric focusing",
abstract = "Humic acids are natural polymers occurring in soils and sediments. These molecules modify the structure of soil and sediment and bind metal ions but because of structural diversity analysis of humic acids is difficult. Capillary isoelectric focusing (CIEF) was first applied in this field by Schmitt et al. but they obtained 3-4 fractions only. The aim of present study was to develop a new and reproducible CIEF method for the separation of humic acids with better resolution. Commercial humic acid samples purchased from Fluka and Aldrich and a peat humic acid were used for method development and comparative studies. Methyl cellulose and polyvinyl alcohol (PVA) were used to increase resolution. Reduced Triton X-100, SDS and urea were applied as anticoagulants. Effects of additives on resolution and reproducibility were compared in different concentrations and optimum concentrations were found to be as follows: 0.2{\%} methyl cellulose, 0.5{\%} PVA and 0.3{\%} reduced Triton X-100. Thus, 30-50 stable fractions could be obtained depending on the sample.",
keywords = "Capillary electrophoresis, Capillary isoelectric focusing, Humic acid",
author = "Piroska Kov{\'a}cs and J{\'o}zsef Posta",
year = "2005",
month = "1",
doi = "10.1016/j.microc.2004.10.012",
language = "English",
volume = "79",
pages = "49--54",
journal = "Microchemical Journal",
issn = "0026-265X",
publisher = "Elsevier Inc.",
number = "1-2",

}

TY - JOUR

T1 - Separation of humic acids using capillary isoelectric focusing

AU - Kovács, Piroska

AU - Posta, József

PY - 2005/1

Y1 - 2005/1

N2 - Humic acids are natural polymers occurring in soils and sediments. These molecules modify the structure of soil and sediment and bind metal ions but because of structural diversity analysis of humic acids is difficult. Capillary isoelectric focusing (CIEF) was first applied in this field by Schmitt et al. but they obtained 3-4 fractions only. The aim of present study was to develop a new and reproducible CIEF method for the separation of humic acids with better resolution. Commercial humic acid samples purchased from Fluka and Aldrich and a peat humic acid were used for method development and comparative studies. Methyl cellulose and polyvinyl alcohol (PVA) were used to increase resolution. Reduced Triton X-100, SDS and urea were applied as anticoagulants. Effects of additives on resolution and reproducibility were compared in different concentrations and optimum concentrations were found to be as follows: 0.2% methyl cellulose, 0.5% PVA and 0.3% reduced Triton X-100. Thus, 30-50 stable fractions could be obtained depending on the sample.

AB - Humic acids are natural polymers occurring in soils and sediments. These molecules modify the structure of soil and sediment and bind metal ions but because of structural diversity analysis of humic acids is difficult. Capillary isoelectric focusing (CIEF) was first applied in this field by Schmitt et al. but they obtained 3-4 fractions only. The aim of present study was to develop a new and reproducible CIEF method for the separation of humic acids with better resolution. Commercial humic acid samples purchased from Fluka and Aldrich and a peat humic acid were used for method development and comparative studies. Methyl cellulose and polyvinyl alcohol (PVA) were used to increase resolution. Reduced Triton X-100, SDS and urea were applied as anticoagulants. Effects of additives on resolution and reproducibility were compared in different concentrations and optimum concentrations were found to be as follows: 0.2% methyl cellulose, 0.5% PVA and 0.3% reduced Triton X-100. Thus, 30-50 stable fractions could be obtained depending on the sample.

KW - Capillary electrophoresis

KW - Capillary isoelectric focusing

KW - Humic acid

UR - http://www.scopus.com/inward/record.url?scp=12344322753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12344322753&partnerID=8YFLogxK

U2 - 10.1016/j.microc.2004.10.012

DO - 10.1016/j.microc.2004.10.012

M3 - Article

AN - SCOPUS:12344322753

VL - 79

SP - 49

EP - 54

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

IS - 1-2

ER -