Separation of enantiomers of β-lactams by HPLC using cyclodextrin-based chiral stationary phases

P. Sun, C. Wang, D. Armstrong, A. Péter, E. Forró

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The enantiomeric separation of 12 β-lactam compounds on 3 native cyclodextrin and 6 derivatized β-cyclodextrin stationary phases was evaluated using high performance liquid chromatography (HPLC). The dimethylphenyl carbamate functionalized chiral stationary phase (CSP) (Cyclobond I 2000 DMP) separated 11 of the 12 β-lactams in the reversed phase mode. The dimethylated β-cyclodextrin column (Cyclobond I 2000 DM) was the second most effective CSP and it separated 8 of the 12 compounds. The reversed phase separation mode was the most effective approach. The effects of the composition and the flow rate on enantioseparations were studied. The effect of the structure of the substituents on the β-lactams was examined.

Original languageEnglish
Pages (from-to)1847-1860
Number of pages14
JournalJournal of Liquid Chromatography and Related Technologies
Volume29
Issue number13
DOIs
Publication statusPublished - Jun 1 2006

    Fingerprint

Keywords

  • Derivatized cyclodextrin-based stationary phase
  • Enantiomer
  • β-Lactam compounds

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

Cite this