Separation of anti-tumor peptides by capillary electrophoresis in organic solvent containing background electrolytes

M. Idei, E. Kiss, Zsófia Dobos, Balázs Hallgas, György Mészŕos, F. Hollósy, G. Kéri

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Connections between the calculated and measured electrophoretic mobilities (μep) determined by capillary electrophoresis as well as connections between the measured and calculated diffusion coefficients of anti-tumor peptides have been investigated in background electrolytes (BGEs) containing different organic solvents (acetonitrile, methanol, ethanol and isopropanol). Comparison of the electrophoretic mobility (μep) values revealed discrepancies between the measured and calculated values. However, no change in the migration order or selectivity could be expected from the calculated μep values, variation of both properties was observed applying organic solvents as BGE modifiers. Experimental determination of the diffusion coefficient suggested that the effect of the organic solvents is not restricted to the change of the BGE viscosity. The reason for the discrepancy between the measured and calculated mobility values might be the possible conformation and/or solvation changes of the peptide caused by the different organic solvents.

Original languageEnglish
Pages (from-to)829-833
Number of pages5
JournalElectrophoresis
Volume24
Issue number5
DOIs
Publication statusPublished - Mar 2003

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Organic solvents
Electrolytes
Tumors
Electrophoretic mobility
Peptides
Neoplasms
2-Propanol
Solvation
Viscosity
Methanol
Conformations
Ethanol

Keywords

  • Anti-tumor peptide
  • Capillary electrophoresis
  • Diffusion coefficient

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Separation of anti-tumor peptides by capillary electrophoresis in organic solvent containing background electrolytes. / Idei, M.; Kiss, E.; Dobos, Zsófia; Hallgas, Balázs; Mészŕos, György; Hollósy, F.; Kéri, G.

In: Electrophoresis, Vol. 24, No. 5, 03.2003, p. 829-833.

Research output: Contribution to journalArticle

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