Separation of active and inactive (nonphosphorylating) Ca2+-ATPase in sacroplasmic reticulum subfractions from low-frequency-stimulated rabbit muscle

Shigeki Matsushita, L. Dux, Dirk Pette

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Chronic low-frequency stimulation elicits in rabbit fast-twitch muscle a partial inactivation of the sarcoplasmic reticulum (SR) Ca2+-ATPase and Ca2+-uptake activities. Inactive Ca2+-ATPase was enriched in a light microsomal fraction by sucrose density gradient centrifugation after calcium oxalate loading in the presence of ATP. This fraction showed a reduced specific activity and phosphoprotein formation of the Ca2+-transport ATPase. These results suggest that the inactivation of the Ca2+-ATPase as induced by increased contractile activity, is confined to a specific SR vesicle population.

Original languageEnglish
Pages (from-to)203-206
Number of pages4
JournalFEBS Letters
Volume294
Issue number3
DOIs
Publication statusPublished - Dec 9 1991

Fingerprint

Reticulum
Calcium-Transporting ATPases
Muscle
Rabbits
Muscles
Sarcoplasmic Reticulum
Calcium Oxalate
Centrifugation
Phosphoproteins
Adenosine Triphosphatases
Sucrose
Density Gradient Centrifugation
Adenosine Triphosphate
Light
Population

Keywords

  • Low-frequency stimulation
  • Sarcoplasmic reticulum Ca-ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Separation of active and inactive (nonphosphorylating) Ca2+-ATPase in sacroplasmic reticulum subfractions from low-frequency-stimulated rabbit muscle. / Matsushita, Shigeki; Dux, L.; Pette, Dirk.

In: FEBS Letters, Vol. 294, No. 3, 09.12.1991, p. 203-206.

Research output: Contribution to journalArticle

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