Separation of 1,4-benzodiazepines by micellar elektrokinetic capillary chromatography

Gabriel Hancu, A. Gáspár, Árpád Gyéresi

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In this work the applicability of micellar elektrokinetic capillary chromatography (MECC) for the determination of benzodiazepines (BZD) has been studied. The applied method was used for the simultaneous separation of 8 BZDs (alprazolam, bromazepam, chlordiazepoxide, diazepam, flunitrazepam, medazepam, oxazepam, nitrazepam), and also for the study of stability in acidic medium. A fast and reliable method has been developed; using a separation buffer composed of sodium tetraborate 25 mM (pH 9.5), SDS (50 mM) and methanol (at least 12%) as an organic modifier.

Original languageEnglish
Pages (from-to)251-259
Number of pages9
JournalJournal of Biochemical and Biophysical Methods
Volume69
Issue number3
DOIs
Publication statusPublished - Jan 10 2007

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Chromatography
Medazepam
Bromazepam
Nitrazepam
Oxazepam
Alprazolam
Flunitrazepam
Chlordiazepoxide
Diazepam
Benzodiazepines
Methanol
Buffers
Bz-423
sodium borate

Keywords

  • Benzodiazepines
  • Capillary electrophoresis
  • Separation
  • Stability

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Separation of 1,4-benzodiazepines by micellar elektrokinetic capillary chromatography. / Hancu, Gabriel; Gáspár, A.; Gyéresi, Árpád.

In: Journal of Biochemical and Biophysical Methods, Vol. 69, No. 3, 10.01.2007, p. 251-259.

Research output: Contribution to journalArticle

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