Separation of κ-opioid receptor subtype from frog brain

József Simon, S. Benyhe, A. Borsodi, M. Szücs, Mária Wollemann

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Complete separation of the [3H]ethylketocyclazocine ([3H]EKC) specific binding (k subtype) from tritiated Tyr-D-Ala2-Me-Phe4-Gly-ol5 enkephalin (DAGO) and Tyr-D-Ala2-L-Leu5-enkephalin (DALA) binding (μ-and δ-subtypes, respectively) was achieved by Sepharose-6B chromatography and sucrose density gradient centrifugation of digitonin solubilized frog brain membranes. The apparent sedimentation coefficient (S20,w) for the k receptor-detergent complex was 13.1 S and the corresponding Stokes radius 64 Å. The isolated fractions exhibited high affinity for EKC and bremazocine, whereas μ- and δ-specific ligands were unable to compete for the [3H]EKC binding sites, indicating that the κ subtype represents a separate molecular entity from the μ and δ receptor sites.

Original languageEnglish
Pages (from-to)395-402
Number of pages8
JournalFEBS Letters
Volume183
Issue number2
DOIs
Publication statusPublished - Apr 22 1985

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Ethylketocyclazocine
Digitonin
Agarose Chromatography
Density Gradient Centrifugation
Enkephalins
Opioid Receptors
Anura
Detergents
Sucrose
Brain
Binding Sites
Ligands
Membranes
Centrifugation
Chromatography
Sedimentation
Sepharose
Ala(2)-enkephalinamide-met
bremazocine

Keywords

  • Gel filtration
  • Hydrodynamical parameters
  • Opioid receptor subtype
  • Separation
  • Solubilization
  • Sucrose density gradient centrifugation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Separation of κ-opioid receptor subtype from frog brain. / Simon, József; Benyhe, S.; Borsodi, A.; Szücs, M.; Wollemann, Mária.

In: FEBS Letters, Vol. 183, No. 2, 22.04.1985, p. 395-402.

Research output: Contribution to journalArticle

Simon, József ; Benyhe, S. ; Borsodi, A. ; Szücs, M. ; Wollemann, Mária. / Separation of κ-opioid receptor subtype from frog brain. In: FEBS Letters. 1985 ; Vol. 183, No. 2. pp. 395-402.
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