Separation and tentative identification of the main pigment fraction of raisins by thin-layer chromatography-fourier transform infrared and high-performance liquid chromatography-ultraviolet detection

Tibor Cserháti, Esther Forgács, Manuel Candeias, Louis Vilas-Boas, Rosario Bronze, Isabell Spranger

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The soluble color pigments of raisin are separated by reversed-phase thin-layer chromatography (TLC), and the capacity of TLC-Fourier transform infrared (FTIR) with both on-line and off-line coupling is assessed for the identification of the main fraction. TLC has also been used as a pilot technique for the development of a gradient elution method for the separation of pigments by high-performance liquid chromatography (HPLC). On-line TLC-FTIR cannot be used for identification because of the strong adsorbance of the stationary phase. Off-line TLC-FTIR combined with the retention behavior of the main pigment fraction indicates that it is a polymer, caramel-like compound composed of erythrose and fructose monomers. Baseline separation of pigments is achieved by HPLC using TLC as a pilot method.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalJournal of chromatographic science
Volume38
Issue number4
DOIs
Publication statusPublished - Apr 2000

ASJC Scopus subject areas

  • Analytical Chemistry

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