Separation and tentative identification of the main pigment fraction of raisins by thin-layer chromatography-fourier transform infrared and high-performance liquid chromatography-ultraviolet detection

T. Cserháti, E. Forgács, Manuel Candeias, Louis Vilas-Boas, Rosario Bronze, Isabell Spranger

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The soluble color pigments of raisin are separated by reversed-phase thin-layer chromatography (TLC), and the capacity of TLC-Fourier transform infrared (FTIR) with both on-line and off-line coupling is assessed for the identification of the main fraction. TLC has also been used as a pilot technique for the development of a gradient elution method for the separation of pigments by high-performance liquid chromatography (HPLC). On-line TLC-FTIR cannot be used for identification because of the strong adsorbance of the stationary phase. Off-line TLC-FTIR combined with the retention behavior of the main pigment fraction indicates that it is a polymer, caramel-like compound composed of erythrose and fructose monomers. Baseline separation of pigments is achieved by HPLC using TLC as a pilot method.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalJournal of Chromatographic Science
Volume38
Issue number4
Publication statusPublished - Apr 2000

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Thin layer chromatography
Vitis
High performance liquid chromatography
Fourier Analysis
Thin Layer Chromatography
Pigments
Fourier transforms
High Pressure Liquid Chromatography
Infrared radiation
Gradient methods
Reverse-Phase Chromatography
Fructose
Polymers
Color
Monomers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

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T1 - Separation and tentative identification of the main pigment fraction of raisins by thin-layer chromatography-fourier transform infrared and high-performance liquid chromatography-ultraviolet detection

AU - Cserháti, T.

AU - Forgács, E.

AU - Candeias, Manuel

AU - Vilas-Boas, Louis

AU - Bronze, Rosario

AU - Spranger, Isabell

PY - 2000/4

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AB - The soluble color pigments of raisin are separated by reversed-phase thin-layer chromatography (TLC), and the capacity of TLC-Fourier transform infrared (FTIR) with both on-line and off-line coupling is assessed for the identification of the main fraction. TLC has also been used as a pilot technique for the development of a gradient elution method for the separation of pigments by high-performance liquid chromatography (HPLC). On-line TLC-FTIR cannot be used for identification because of the strong adsorbance of the stationary phase. Off-line TLC-FTIR combined with the retention behavior of the main pigment fraction indicates that it is a polymer, caramel-like compound composed of erythrose and fructose monomers. Baseline separation of pigments is achieved by HPLC using TLC as a pilot method.

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