Separation and quantitation of colour pigments of chili powder (Capsicum frutescens) by high-performance liquid chromatography-diode array detection

Tibor Cserháti, Esther Forgács, Maria Helena Morais, Teresa Mota, Ana Ramos

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The performance of reversed-phase thin-layer (RP-TLC) and reversed-phase high-performance liquid chromatography (RP-HPLC) was compared for the separation and determination of the colour pigments of chili (Capsicum frutescens) powder using a wide variety of eluent systems. No separation of pigments was achieved in RP-TLC, however, it was established that tetrahydrofuran shows an unusually high solvent strength. RP-HPLC using water-methanol-acetonitrile gradient elution separated the chili pigments in many fractions. Diode array detection (DAD) indicated that yellow pigments are eluted earlier than the red ones and chili powder contains more yellow pigments than common paprika powders. It was established that the very different absorption spectra of pigments make the use of DAD necessary. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalJournal of Chromatography A
Volume896
Issue number1-2
DOIs
Publication statusPublished - Oct 27 2000

Keywords

  • Capsicum frutescens
  • Food analysis
  • Pigments

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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